大鼠孕期慢性应激对子鼠学习记忆能力及海马胆碱能神经递质的影响  

Effect of Maternal Chronic Stress During Pregnancy on the Learning and Memory Ability and Hippocampal Cholinergic Neurotransmitter of Offspring Rats

作　　者：关素珍[1] 李榕[2] 徐仙[2] 刘继文[2] 

机构地区：[1]新疆医科大学公共卫生学院社会医学教研室,新疆乌鲁木齐830011 [2]新疆医科大学公共卫生学院劳动卫生与环境卫生学教研室,新疆乌鲁木齐830011

出　　处：《环境与职业医学》2016年第12期1133-1137,共5页Journal of Environmental and Occupational Medicine

基　　金：新疆维吾尔自治区青年科学基金(编号:2013211B50)

摘　　要：[目的]观察大鼠孕期处于慢性应激状态时子鼠学习记忆能力的变化,从海马胆碱能神经递质方面探讨损伤的可能机制。[方法]建立孕期慢性应激(CUMS)大鼠模型,采用放射免疫法测定母鼠血浆皮质酮水平,利用Morris水迷宫和Y迷宫进行子鼠学习记忆能力的测定,采用ELISA方法测定脑海马组织胆碱能神经递质含量,并分析其相关性。[结果]模型组母鼠皮质酮水平与对照组的差异有统计学意义(F=12.347,P=0.001),提示模型组大鼠处于应激状态。模型组子鼠的体重在出生后第28天和出生后第42天均低于对照组(P<0.05);模型组子鼠血浆皮质酮水平高于对照组(P<0.05)。子鼠学习记忆能力变化:模型组子鼠逃避潜伏期时间比对照组子鼠长(F=8.579,P<0.001),跨平台次数低于对照组(t=3.344,P=0.001);模型组子鼠学习所需的训练次数高于对照组子鼠,而记忆保持测试的正确反应率低于对照组子鼠(均P<0.05)。与对照组子鼠相比,模型组子鼠乙酰胆碱含量、胆碱乙酰转移酶活性下降,乙酰胆碱酯酶活性上升(均P<0.05)。子鼠的学习记忆指标与血浆皮质酮及海马组织胆碱能神经递质水平存在相关性(均P<0.05)。[结论]孕期慢性应激影响子鼠学习记忆能力,与子鼠血浆皮质酮及海马组织胆碱能神经递质水平的变化有关。[Objective] To observe the changes of learning and memory ability of offspring rats following maternal chronic stress during pregnancy, and study the possible mechanisms of injury related with hippocampal cholinergic neurotransmitter. [Methods] A rat model was set up receiving chronic unpredictable mild stress（CUMS） during pregnancy. Plasma corticosterone levels of maternal rats were determined by radioimmunoassay. Learning and memory ability were tested using Morris water maze and Y-maze experiments. The levels of hippocampal cholinergic neurotransmitters were detected by ELISA for all experimental animals. The correlation between learning and memory ability and hippocampus cholinergic neurotransmitters was analyzed. [Results] An elevation of the plasma corticosterone level was observed in the model rats（F=12.347, P=0.001）, indicating stress state. The body weight of the model rats＇ offsprings was lower while the plasma corticosterone level was higher than the corresponding indicators of the control group＇s offsprings in postnatal day（PND） 28 and PND 42（P〈0.05）. In terms of learning and memory ability, the escape latency of the model rats＇ offsprings was longer than that of the control group＇s offsprings（F=8.579, P〈0.001）. The times of crossing platform were fewer of the model rats＇ offspring than those of the control group＇s offspring（t=3.344, P=0.001）. The times of required training was higher while the rate of correct response in model rats＇ offsprings was lower than that of the control group＇s offsprings（both Ps〈0.05）. Decreased Ach content and Ch AT activity and elevated Ach E activity of the model rats＇ offsprings were found, compared with the control group＇s offsprings（all Ps〈0.05）. There were correlations of learning and memory ability with plasma corticosterone and hippocampal cholinergic neurotransmitters of offspring rats（both Ps〈0.05）. [Conclusion] The learning and memory ability is related to plasma corticosterone and hippocampal chol

关 键 词：孕期 应激 子鼠 学习记忆 海马 胆碱能神经递质 

分 类 号：R131[医药卫生—劳动卫生]