乌司他丁、氨溴索肺灌洗对百草枯致兔肺损伤的疗效观察  

Comparative Study on Curative Effects of Pulmonary Perfusion with Ulinastatin and Ambroxol on Lung Injury Induced by Paraquat in Rabbits

作　　者：史文佩 张斐斐[1] 陈立章[1] 王安[1] 

机构地区：[1]中南大学公共卫生学院,湖南长沙410000

出　　处：《环境与职业医学》2016年第12期1152-1156,共5页Journal of Environmental and Occupational Medicine

基　　金：中南大学米塔尔学生创新项目(编号:12MX22);中南大学本科生自由探索计划项目(编号:2282013bks069)

摘　　要：[目的]比较不同肺灌洗液对百草枯(PQ)致兔肺损伤的治疗效果。[方法]30只家兔随机分为5组:百草枯染毒组、生理盐水灌洗组、氨溴索灌洗组、乌司他丁灌洗组及对照组,每组6只动物。前4组一次性灌胃50 mg/kg体重的百草枯,3个灌洗组在染毒24 h后,分别给予生理盐水、氨溴索、乌司他丁进行肺灌洗;对照组一次性灌胃等体积的生理盐水,24 h后再行生理盐水肺灌洗。采集各组动物染毒前、染毒后第1、3、7天血液进行血清学检测;染毒后第7天处死动物,取其右肺组织进行病理学观察。[结果]染毒后第3、7天各灌洗组血清髓过氧化物酶(MPO)、羟脯氨酸(HYP)、白介素-1(IL-1)、肿瘤坏死因子-α(TNF-α)均低于百草枯染毒组(均P<0.05)。染毒后第7天,氨溴索灌洗组血清MPO及IL-1含量低于生理盐水灌洗组(均P<0.05);氨溴索灌洗组HYP水平高于乌司他丁灌洗组(P<0.05),但低于生理盐水灌洗组(P<0.05);乌司他丁灌洗组TNF-α水平低于氨溴索灌洗组(P<0.05)。3个灌洗组肺组织病理改变较染毒组轻。[结论]肺灌洗疗法可降低家兔百草枯中毒所致急性肺损伤的炎症反应,乌司他丁灌洗及氨溴索灌洗效果优于单纯生理盐水灌洗。[Objective] To compare the curative effects of selected lung lavage fluids on rabbits＇ lung injury induced by paraquat（PQ）. [Methods] Thirty rabbits were randomly assigned to PQ group, normal saline group, ambroxol group, ulinastatin group, and control group, with six rabbits per group. Rabbits of the exposure groups were intragastrically administered once with PQ at 50 mg/kg; the normal saline, ambroxol, and ulinastatin groups were treated by lung lavage with respective agents after 24 h exposure of PQ; animals in the control group were administered with 50 mg/kg physiological saline and treated by lung lavage with saline after 24 h. Before exposure and at 1, 3 7 d after exposure, rabbit auricular vein blood was sampled for serum examination. The animals were executed on the 7th day after exposure and right lung tissues were sampled for pathological observation. [Results] On the 3rd and 7th day after exposure, the levels of serum myeloperoxidase（MPO）, hydroxyproline（HYP）, interleukin 1（IL-1）, and tumor necrosis factor α（TNF-α） of the normal saline, ambroxol, and ulinastatin groups were significantly lower than those of the PQ group（all Ps 0.05）. On the 7th day after exposure, the MPO level and IL-1 level were lower in the ambroxol group than those in the normal saline group（both Ps〈0.05）; the HYP level in the ambroxol group was higher than that in the ulinastatin group（P〈0.05）, but lower than that in the normal saline group（P〈0.05）; the TNF-α level in the ulinastatin group was lower than that in the ambroxol group（P〈0.05）. Milder pathological changes were found in the three lung lavage groups than those in the PQ group. [Conclusion] Lung lavage might have protective effects on the inflammatory response of acute lung injury caused by PQ in rabbits. Lung lavage with ambroxol or ulinastatin is better than using physiological saline alone.

关 键 词：百草枯 肺灌洗 急性肺损伤 乌司他丁 氨溴索 

分 类 号：R114[医药卫生—卫生毒理学]