降解菌Pseudomonas sp.对阿特拉津的降解条件优化  

Optimization for Degradation Conditions of a Pseudomonas sp.Strain with Degrading Atrazine Ability

作　　者：刘丹丹[1] 刘畅[1] 刘长风[1] 李欣燕[1] 

机构地区：[1]沈阳化工大学环境与安全工程学院,辽宁沈阳110142

出　　处：《贵州农业科学》2016年第10期46-49,共4页Guizhou Agricultural Sciences

基　　金：辽宁省博士科研启动项目"土壤阿特拉津污染的微生物修复机制与工程菌株构建"(20141086)

摘　　要：为微生物降解菌在农药污染土壤修复工程中应用提供参考,以从长期受阿特拉津污染的农田土壤中筛选出的一株降解菌Pseudomonas sp.为研究对象,采用单因素试验研究其在不同培养条件下对阿特拉津的降解效果,并采用正交试验进一步优化该菌的降解条件。结果表明：碳源对降解效果的影响不大;培养时间48h,底物浓度100mg/L,温度25~35℃,pH 5.0~8.0,盐度0.1%~1%时,该菌对阿特拉津的降解效果较好,降解率大于90%;该菌对阿特拉津降解的最佳组合条件为温度30℃,pH 7.0,盐度0.5%;且3因素对降解效果的影响依次为温度〉pH〉盐度。The degrading Atrazine effect of a Pseudomonas sp.strain screened from the soil polluted with Atrazine for long time was studied under different culture conditions by the single-factor test and the degradation conditions were optimized by the orthogonal test to provide a reference for application of microbial degradation bacteria in restoration works of soil polluted with pesticides.Results：Carbon source has no obvious effect on degradation effect.The Atrazine degradation rate of the Pseudomonas sp.strain is more than 90% under the culture conditions of 48 h,100 mg/L substrate concentration,25~35℃,pH5.0~8.0and 0.1% ~1% salinity.The optimal condition combination of the Pseudomonas sp.strain includes 30℃,pH 7.0and 0.5% salinity and the influencing degree is temperature〉 pH 〉 salinity.

关 键 词：PSEUDOMONAS sp. 微生物降解菌 阿特拉津 农药残留 环境污染 降解力 

分 类 号：S154.39[农业科学—土壤学] X172[农业科学—农业基础科学]