黑质致密部和内侧前脑束注射6-OHDA制备的帕金森病大鼠模型纹状体中DA含量比较  

Effects of 6-OHDA-lesioned Parkinson's disease rat model in substantia nigra compacta and medial forebrain bundle on DA level in the striatum

作　　者：韩玲娜[1] 常永丽[1] 郭晓姝[1] 张翠英[1] 

机构地区：[1]长治医学院生理学教研室,046000

出　　处：《长治医学院学报》2016年第5期334-337,共4页Journal of Changzhi Medical College

基　　金：长治医学院科技创新团队项目(CX201506)

摘　　要：目的:比较黑质致密部(SNc)损毁和内侧前脑束(MFB)损毁2种方法制备的帕金森病(PD)大鼠模型对纹状体中多巴胺(DA)递质含量的影响。方法:将大鼠随机分为假手术组(n=12)、SNc损毁组(n=15)和MFB损毁组(n=14)。采用高效液相色谱-电化学检测法,观察3组大鼠损毁侧纹状体中DA的含量。结果:与假手术组相比较,SNc损毁组(P<0.001)和MFB损毁组(P<0.001)大鼠纹状体中DA含量均显著降低,与SNc损毁组相比较,MFB损毁组大鼠纹状体中DA含量下降更为显著(P=0.005)。结论:MFB损毁制备的PD大鼠模型对DA能神经元的损伤范围较SNc损毁有所扩大,为不同研究选择制备模型的方法提供一定的理论依据。Objective：To study the effects of substantia nigra compacta （SNc）lesioned and medial forebrain bundle （MFB）lesion Parkinson’s disease（PD）rat models by 6-hydroxydopamine （6-OHDA）on the dopamine （DA）level in the striatum.Methods：Rats were randomly divided into sham-operated （n = 12）,SNc-lesioned（n = 1 5 ）and MFB-lesioned （n =14）groups.Changes of the DA level in the striatum were observed by the high performance liquid chromatography with elec-trochemical detection in 3 groups rats.Results：Compared to sham-operated rats,the DA levels of the SNc-lesioned and MFB-lesioned rats decreased significantly.And compared to the SNc-lesioned rats,the degree of DA levels in the MFB-lesioned rats docreased more serious （P =0.005）.Conclusion：The lesioned range of DA neurons of MFB-lesioned PD rat model is wider than that of SNc-lesion,which provides a theory basis for the choice of modeling methods in different researches.

关 键 词：帕金森病大鼠模型 黑质致密部 内侧前脑束 

分 类 号：R331[医药卫生—人体生理学]