Characteristic Analysis of EST-SSR Markers in Lettuce(Lactuca sativa)  

生菜EST-SSR的分布特征分析（英文）

作　　者：张光望 游杨[1] 罗延延 张明菊[1,2,3] 李志良[1,2,3] 王书珍[1,2,3] 

机构地区：[1]黄冈师范学院生命科学学院,湖北黄冈438000 [2]大别山特色资源开发湖北省协同创新中心,湖北黄冈438000 [3]经济林木种质改良与资源综合利用湖北省重点实验室,湖北黄冈438000

出　　处：《Agricultural Science & Technology》2016年第12期2682-2685,2688,共5页农业科学与技术（英文版）

基　　金：Supported by General Project of Natural Science Foundation of Hubei Province(2015CFC816)~~

摘　　要：From the NCBI database, 81,518 Lactuca sativa EST sequences were downloaded, from which 61,757 non-redundant sequences were obtained. In total, 2040 SSR loci were identified, with the frequency of 3.3%. Trinucleotide repeat was dominant, followed by dinucieotide and hexanucleotide repeats. Among 181 types of repeat motifs, （AGA）n and （ATG）n were the dominant repeat motif types, taking up 12.01% and 3.53%, respectively. Totally, four material-special EST-SSR markers were characterized. This study enriches molecular markers of L. sativa, and also lays a theoretical foundation for following germplasm identification, molecular marker assisted breeding and genetic map construction.从NCBI数据库下载了81 518条生菜EST序列,处理后得到无冗余序列61 757条,共搜索到2 040个SSR位点,出现频率为3.3%。三碱基重复是主要的类型,占38.53%,其次是二碱基和六碱基重复,出现次数最低的是四碱基重复。在181种重复基元类型中,(AGA)n和(ATG)n是主导的重复基元类型,分别占12.01%和3.53%。设计合成的30对引物在50份生菜资源进行鉴定,共筛选出4个材料特异性标记。该研究为后续开发生菜多态性EST-SSR标记,进行种质资源鉴定、分子标记辅助育种、遗传图谱构建奠定了基础。

关 键 词：L-actuca-sativa Expressedsequence tag MICROSATELLITE Germplasm re-source 

分 类 号：S636.2[农业科学—蔬菜学]