植物提取物碳酸酐酶对肾结石大鼠的影响  

Effects of carbonic anhydrase extracts from plant on renal calculi rats

作　　者：刘小良[1,2] 廖鑫鑫[1,2] 黄鹏[1,2] 周海滨[1,2] 宋秋元[1,2] 曾泉[1,2] 魏胜红[1,2] 付强[1,2] 陆文宝[1,2] 黄玉清[1,2] 张良[1,2] 唐青[1,2] LIU Xiao-liang LIAO Xin-xin HUANG Peng ZHOU Hai-bin SONG Qiu-yuan ZENG Quan WEI Sheng-hong FU Qiang LU Wen-bao HUANG Yu-qing ZHANG Liang TANG Qing(Department of Urology, the Affiliated Hospital of Jiujiang University, Jiujiang 332000, Jiangxi Province, China Scientific Research Center, the Affiliated Hospital of Jiujiang University, Jiujiang 332000, Jiangxi Province, China)

机构地区：[1]九江学院附属医院泌尿外科,江西九江332000 [2]九江学院附属医院科研中心,江西九江332000

出　　处：《新乡医学院学报》2016年第1期12-15,共4页Journal of Xinxiang Medical University

基　　金：九江市科技支撑计划项目(编号:2014161009)

摘　　要：目的观察碳酸酐酶(CA)对肾结石大鼠的影响。方法从植物叶片中提取CA并检测活性。将40只大鼠随机分为Ⅰ、Ⅱ、Ⅲ和Ⅳ组,每组25只。Ⅰ组大鼠正常喂养颗粒饲料;Ⅱ组大鼠每天给予质量分数1%乙二醇+质量分数2%氯化铵2 m L灌胃;Ⅲ组大鼠每天给予质量分数1%乙二醇+质量分数2%氯化铵2 m L+CA 1 m L灌胃;Ⅳ组大鼠每天给予CA 1 m L灌胃。饲养1周后收集4组大鼠24 h尿量,并检测尿液的尿素氮、肌酐、尿酸及钙离子。30 d后处死大鼠,观察肾脏组织结晶形成情况。结果 4组大鼠尿液中的尿素氮、肌酐及尿酸水平比较差异均无统计学意义(P>0.05);Ⅲ、Ⅳ组大鼠24 h尿量与Ⅰ、Ⅱ组比较差异有统计学意义(P<0.05);4组大鼠尿钙两两比较差异均有统计学意义(P<0.05)。Ⅱ组可见明显的肾结石结晶;Ⅲ、Ⅳ组未见明显的肾结石结晶,但可见肾小管结构异常改变。结论植物提取物CA能够抑制大鼠肾结晶的形成,对肾结石的预防和治疗可能有一定的积极作用。Objective To explore the effect of carbonic anhydrase （CA） on the animal model of renal calculi rats. Methods The CA was extracted from plant and its activity was detected. Forty rats were divided into four groups ,with twentyfive rats in each group. The rats in group Ⅰ were fed pelleted diet;the rats in group Ⅱ were fed quality concentration 1% ethylene glycol ＋ quality concentration 2% ammonium chloride 2 mL;the rats in group Ⅲ were fed quality concentration 1% ethylene glycol ＋ quality concentration 2% ammonium chloride 2 mL ＋ CA 1 mL;the rats in group Ⅳ were fed CA 1 mL. All rats were oral administration per day for 30 days. A week after feeding ,24 h urine volume was collected, and the urea nitrogen, creatinine, uric acid and calcium was detected. One month later, rats were sacrificed and the kidney tissues crystal were observed. Results There was no statistic difference of urine urea nitrogen, creatinine and uric acid levels of rats in the four groups （ P 〉 O. 05 ） ; there was statistic difference of 24 h urine volume between group Ⅲ, group Ⅳ and group I , group U （ P 〈 0.05 ） ; there was statistic difference of calcium oxalate in urine among the four groups （ P 〈 0.05 ）. After rats were sacrificed, renal crystal was observed only in group Ⅱ ; no obvious renal crystal was observed in group Ⅲ and group Ⅳ,but abnormal structure of renal tubule was observed. Conclusion Plant extraction CA can inhibit the formation of renal crystal, may have certain positive role in prevention and treatment of renal calculi.

关 键 词：碳酸酐酶 肾结石 动物模型 尿排泄 

分 类 号：R692.4[医药卫生—泌尿科学]