木香对溃疡性结肠炎大鼠干预作用的影响  

Protective effect of Radix Aucklandiae against ulcerative colitis in rats

作　　者：刘海荣[1] 马露[1] 唐方[1] 张勇[1] 杨瑾 

机构地区：[1]天津医科大学总医院中医科,天津市300052 [2]天津中新药业集团有限公司乐仁堂制药厂,天津市300193

出　　处：《世界华人消化杂志》2016年第33期4467-4473,共7页World Chinese Journal of Digestology

基　　金：天津市科委科技创新专项基金资助项目;No.06FZZDSH00405~~

摘　　要：目的通过探讨木香对溃疡性结肠炎(ulcerative colitis,UC)大鼠的干预作用机制,阐释胃肠安丸"芳香化浊"药物作用靶点.方法清洁级Wistar♂大鼠40只随机分为4组,分别为空白对照组(N)、模型组(C)、中药饮片高、低剂量组(MX20、MX10)每组10只,采用2,4,6-三硝基苯磺酸/乙醇混合液灌肠法诱导实验性UC,空白对照组和模型组以蒸馏水灌胃,中药组以木香水煎剂灌胃,2 m L/只,1次/d,连续7 d.观察UC大鼠疾病活动指数(disease activity index,DAI)、结肠黏膜损伤指数(colon macroscopic damage index,CMDI)及组织病理学变化、血清超氧化物歧化酶(superoxide dismutase,SOD)、血浆丙二醛(malondialdehyde,MDA)、前列腺素E2(prostaglandin E2,PGE2)、表皮生长因子(epidermal growth factor,EGF)含量及结肠肠三叶因子(intestinal trefoil factor,TFF3)表达.结果木香可有效减轻UC大鼠腹泻次数及黏液脓血便,降低DAI、CMDI积分.光镜下可见病变结肠上皮呈不同程度修复,溃疡面积缩小.血清SOD、EGF、结肠TFF3含量显著升高,血浆MDA、PGE2含量显著性降低.结论木香对UC的干预作用是胃肠安丸芳香化浊作用机制的科学阐释.AIM To investigate whether Radix Aucklandiae has a protective effect against ulcerative colitis in rats to explain the action target of aromatic drugs in Weichangan pill.METHODS Forty male Wistar rats were randomly and equally divided into four groups： normal control group, 2,4,6-trinitrobenzenesulfonic acid（TNBS） group, high dosage Radix Aucklandiae（MX20） group, and low dosage Radix Aucklandiae（MX10） group. Experimental colitis was induced in rats with TNBS and ethanol. The rats in the normal and TNBS groups were given distilled water by intragastric administration, while the rats in the MX20 and MX10 groups were given Radix Aucklandiae by intragastric administration, once a day, for 7 d. Disease activity index（DAI）, colon macroscopic damage index（CMDI）, histopathological changes, serum superoxide dismutase（SOD）, plasma malondialdehyde（MDA）, prostaglandin E2（PGE2）, and epidermal growth factor（EGF） contents, and intestinal trefoil factor（TFF3） expression in the colon were compared between different groups.RESULTS Radix Aucklandiae significantly reduced the incidence of diarrhea and mucous, purulent, bloody stool and lowered DAI and CMDIscores compared with the TNBS group. After Radix Aucklandiae treatment, varying degrees of repair of lesions in the colon epithelium occurred and ulcer area became narrower under a light microscope. In addition, Radix Aucklandiae increased the contents of SOD, EGF, and TFF3, and lowered the contents of MDA and PGE2 signifi cantly.CONCLUSION Protective effect of Radix Aucklandiae against ulcerative colitis in rats interprets the effi cacy of aromatic drugs in Weichangan pill.

关 键 词：溃疡性结肠炎 木香 胃肠安丸 大鼠 

分 类 号：R285.5[医药卫生—中药学]