CRTH2受体拮抗剂对支气管哮喘树突状细胞功能的影响  

Effects of CRTH2 receptor antagonist on the function of dendritic cells in patients with bronchial asthma

作　　者：陈娜 张双 高春燕 CHEN Na ZHANG Shuang GAO Chunyan(Department of Internal Pediatrics, Tangshan Maternal and Children Care Hospital, Tahngshan 063000, China Department of Clinical Laboratory, Tangshan Maternal and Children Care Hospital, Tahngshan 063000, China)

机构地区：[1]唐山妇幼保健院儿内科,063000 [2]唐山妇幼保健院检验科,063000

出　　处：《免疫学杂志》2017年第1期63-67,共5页Immunological Journal

摘　　要：目的培养支气管哮喘患儿外周血树突状细胞,体外给予CRTH2受体拮抗剂干预,从而探讨CRTH2受体在支气管哮喘发病中的作用。方法提取外周血单个核细胞,体外给于10 ng/ml GM-CSF及IL-4的完全培养液培养,并在第5天磁珠分选得到纯度90%以上的树突状细胞,体外给予CRTH2受体拮抗剂干预,收集细胞及上清,运用流式细胞技术检测DCs表型,ELISA方法检测细胞上清IL-10、IL-12含量变化,同种混合淋巴细胞反应检测树突状细胞刺激淋巴细胞增殖及分化的能力。结果经磁珠分选后,树突状细胞纯度可达90%,符合实验要求;给药组与对照组相比,给药组外周血树突状细胞表面表达的CD80、CD86、CD40显著增高(P<0.05);给药组外周血树突状细胞分泌的IL-12明显高于对照组,IL-10明显低于对照组(P<0.05);给药组外周血树突状细胞刺激T淋巴细胞增殖能力显著大于对照组(P<0.05);给药组外周血树突状细胞刺激T淋巴细胞分泌IL-4含量明显低于对照组,IFN-γ含量明显高于对照组(P<0.05)。结论与对照组相比,CRTH2受体可能通过调节树突状细胞的功能来达到参与疾病的发生发展。To investigate the roles of CRTH2 in bronchial asthma, dendritic ceils （DCs） from patients with bronchial asthma were cultured with the antagonist of CRTH2 receptor in vitro. Mononuclear cells were extracted from peripheral blood of patient, and then cultured in complete medium （10 ng/ml GM-CSF, IL-4） for 5 days. After magnetic cell sorting, the DCs were intervened with the antagonist of CRTH2 receptor. Fluorescence-activated cell sorting analysis was applied to detect the expression of costimulatory molecules; enzyme-linked immunosorbent assay was used to detect cytokine production of IL-10 and IL-12; allogeneic mixed lymphocyte reaction was utilized to detect the ability of DCs to stimulate T cells. Data showed that the purity of DCs was about 90% after magnetic cell sorting. Compared with the control group, experimental group showed higher levels of IL-12, CD80, CD86, CD40, but lower level of IL-10 （P〈0.05）. Further more, DCs from experimental group displayed higher ability to induce T cell proliferation （P〈0.05）, and could induced T cell to secret higher level of IFN-γand lower level of IL-4 （P〈0.05）, as compared with control group. In conclusion, CRTH2 receptor is involved in the development and progression of bronchial asthma by modulating the function of DCs.

关 键 词：树突状细胞 支气管哮喘 成熟 CRTH2受体 

分 类 号：R562.25[医药卫生—呼吸系统]