花生Clp家族成员的筛选、聚类和盐胁迫响应分析  

Screening,Clustering and Response to Salinity Stress of Clp Family Genes in Peanut( Arachis hypogaea L.)

作　　者：郑春花[1] 孔祥远[2] 陈冠旭[2] 隋炯明[2] 乔利仙[2] 王晶珊[2] 赵春梅[2] 

机构地区：[1]青岛农业大学图书馆,山东青岛266109 [2]青岛农业大学生命科学学院,山东青岛266109

出　　处：《山东农业科学》2016年第12期1-5,共5页Shandong Agricultural Sciences

基　　金：国家自然科学基金项目(31571705;31301356;31471542);山东省科技发展计划项目(2014GNC110002)

摘　　要：Clp蛋白酶是一种ATP依赖的丝氨酸型蛋白酶,广泛存在于原核和真核生物中,在生物抗逆胁迫中发挥重要作用。为分析花生中Clp基因的情况,我们构建了花生叶片转录组数据库,通过生物信息学手段鉴定出28个Clp基因,分别位于花生A组野生种的9条染色体上;聚类分析表明,花生的28个Clp基因分别聚到已报道的Clp B、C、D、X、P、R和S 7个亚类中。利用花生耐盐突变体(S2)和对照(S4)构建了盐胁迫处理前后各时间段的表达谱数据,进行Clp基因盐胁迫表达分析,结果表明16个Clp基因在S2和(或)S4中受盐胁迫诱导表达。该研究为花生Clp基因的功能研究与利用奠定基础。Clp is a kind of ATP-dependent protease widespreading in prokaryote and eukaryote,which plays an important role in resisting biotic and abiotic stresses in plants. To improve the understanding of Clp genes in peanut,28 Clp genes were screened by one transcriptome library constructed with peanut leaves.These 28 Clp genes distributed on 9 chromosomes respectively of A genome of wild peanut. Clustering analysis showed that these 28 Clp genes were assorted to Clp B,C,D,X,P,R,S groups reported previously. To analyze the response to salinity stress of 28 Clp genes,digital gene expression profiles were constructed using a mutant with higher salinity resistance（ S2） and its control（ S4） before and after treated with 250 mmol / L Na Cl. The results showed that 16 Clp genes were responsive to salinity stress in S2 and / or S4. This study provided bases for functional researches and application of Clp genes in peanut.

关 键 词：花生 Clp基因 RNA测序 盐胁迫 

分 类 号：S565.203.53[农业科学—作物学]