一个猪场高致病性PRRSV的分离鉴定  

Isolation and Identification of a Highly Pathogenic PRRSV from a Pig Farm

作　　者：任素芳[1] 郭立辉[1] 李为栋 张召坤[1] 李兴光[1] 崔宝玉 吴家强[1] 张玉玉[1] 

机构地区：[1]山东省农业科学院畜牧兽医研究所/山东省畜禽疫病防治与繁育重点实验室,山东济南250100 [2]山东农业大学动物科技学院,山东泰安271018 [3]山东畜牧兽医职业学院,山东潍坊261061

出　　处：《山东农业科学》2016年第12期129-132,共4页Shandong Agricultural Sciences

基　　金：国家自然科学基金项目(31572550);山东省现代农业产业技术体系项目(SDAIT-06-022-06);山东省农业科学院重大科技成果培育计划项目(2014CGPY04);泰山学者工程项目

摘　　要：本研究对一个猪场高致病性PRRSV疑似病例进行了病理剖检、PRRSV N蛋白基因RT-PCR扩增以及序列测定和分析。结果表明,N2号分离株N蛋白核苷酸序列与高致病性毒株(JXA1株、Hu N4株、SX-1株、TJM株)的一致性均为99.1%,N3/N4号与高致病性毒株的一致性均为98.9%,由此判定该猪场病猪感染毒株为高致病性PRRSV。该研究结果可为养猪生产中高致病性PRRSV感染的判断提供借鉴。The suspected cases of highly pathogenic PRRSV from a pig farm were conducted autopsy,the gene sequence of N-protein of PRRSV was amplified by RT-PCR,and then its sequence was determined and analyzed. The identity of the nucleotide sequence of N-protein was 99. 1% between the isolate N2 and the highly pathogenic strains（ JXA1,Hu N4,SX-1 and TJM）,and that of isolate N3 / N4 was 98. 9% with the highly pathogenic strains. The results showed that the infected virus was highly pathogenic PRRSV in the pig farm. This research provided a reference for the diagnosis of highly pathogenic PRRSV infection in swine production.

关 键 词：病理解剖 PRRS 高致病性PRRSV RT-PCR 序列测定 

分 类 号：S858.285.3[农业科学—临床兽医学]