正交试验法优选鳢肠总黄酮提取工艺  

Optimization of Extraction Process for Total Flavonoids from Eclipta prostrata L.

作　　者：颜峻[1] 胡柳芝 王文彬[1] 谢中国[1] 邓爱华[1] 

机构地区：[1]湖南文理学院生命科学学院,水产高效健康生产湖南省协同创新中心,环洞庭湖水产健康养殖及加工湖南省重点实验室,动物学湖南省高校重点实验室,湖南常德415000

出　　处：《安徽农业科学》2016年第34期125-127,169,共4页Journal of Anhui Agricultural Sciences

基　　金：湖南省“十二五”重点建设学科(动物学);湖南省高校科技创新团队项目“水生生物资源保育与利用”;湖南文理学院“范蠡”本科创新人才培养计划项目

摘　　要：[目的]优化鳢肠总黄酮的提取工艺。[方法]采用单因素试验和正交试验法,以总黄酮提取率为评价指标,研究了乙醇浓度、提取时间、提取温度、溶剂用量4种因素对总黄酮提取的影响。[结果]各因素对鳢肠总黄酮提取率的影响从大到小依次溶剂用量、乙醇浓度、提取时间、提取温度;最佳提取工艺为乙醇浓度40%、提取时间2 h、提取温度80℃、溶剂用量25倍,此条件下鳢肠总黄酮平均提取率达71.01%。[结论]该提取工艺简单可行,具有良好的稳定性和重复性,可为鳢肠总黄酮的工业化生产提供参考。[ Objective ] The research aimed to optimize the extraction process of total flavonoids of Eclipta prostrata L.. [ Method ] Using single factor test and orthogonal test,the extraction rate of total flavonoids was taken as the evaluation index,the effects of ethanol concentration, ex- traction time, extraction temperature and solvent dosage on the extraction of total flavonoids were studied. [ Result] The effects of various factors on the extraction rate of total flavonoids from big to small were as follows： solvent dosage, ethanol concentration, extraction time, extraction temperature. The optimum extraction conditions were ethanol concentration 40%, extraction time 2 h, extraction temperature 80 ℃ and solvent dosage 25 times. The average extraction rate of total flavonoids was 71. 01 %. [ Conclusion ] The extraction process is simple and feasible, it has good stability and repeatability, which can provide reference for the industrial production of total flavonoids of Eclipta prostrata L..

关 键 词：鳢肠 总黄酮 正交试验 提取工艺 

分 类 号：S567.219[农业科学—中草药栽培]