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出 处:《西部中医药》2016年第11期30-32,共3页Western Journal of Traditional Chinese Medicine
摘 要:目的:通过HPLC法比较川楝子不同炮制品中川楝素和异川楝素含量的差异。方法:采用HPLC法对川楝子生品及各炮制品,以川楝素和异川楝素作为指标,进行含量测定。色谱条件:色谱柱(4.6 mm×250 mm,5μm);流动相:乙腈-水(30∶70);检测波长210 nm;柱温35℃;进样量10μL;流速1.0 m L/min。结果:炒品相对其他炮制品种而言,川楝素和异川楝素含量较高。结论:不同炮制方法中,炒品中川楝素和异川楝素含量下降幅度最小。Objective: To compare and analyze the toosendanin and isotoosendanin content in different processed ChuanLianZi by HPLC method. Methods: toosendanin and isotoosendanin content level in raw and different prepared ChuanLianZi were took as index and tested by HPLC method. The chromatographic conditions: chromatographic column(4.6 mmx250 nun, 5 μm), mobile phase: Acetonitrilewater (30:70), determine wavelength was 210 nm, column temperature was 35℃, sample size was 10 μL, flow velocity was 1.0 mL/min. Results: Toosendanin and isotoosendanin content were higher in fried ChuanLianZi than others. Conclusion: Toosendanin and isotoosendanin content decreased minimum in fried ChuanLianZi among the different processed methods.
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