土壤中汞胁迫对牧草生理生化的影响  

The influence of mercury in the soil stress on physiological characteristics of forage

作　　者：王道涵[1] 杜俊楠[1] 李玉娥[1] 初世敬 

机构地区：[1]辽宁工程技术大学环境科学与工程学院,阜新123000

出　　处：《地球环境学报》2016年第6期605-612,共8页Journal of Earth Environment

基　　金：(美国)国家地理空气与水保护基金资助项目(GEFC30-14)~~

摘　　要：研究紫花苜蓿、披碱草、高羊茅三种牧草,在10个浓度梯度汞胁迫条件下发芽率、植株高度、过氧化物酶与过氧化氢酶活性、丙二醛与脯氨酸含量、可溶性蛋白及可溶性糖含量生理生化指标的变化情况。旨在探索土壤中汞对牧草的影响机理,反映土壤中汞含量富集过多后对草原的影响。Background, aim, and scope Parts of large-scale coal enterprises in Inner Mongolia have been striving to develop coal-fired power plants and promoting the integration of coal and electricity, which indicates the rapid increase of coal consumption in the region of Inner Mongolia. During the coal combustion, the phenomenon of mercury emissions into the atmosphere appears, which has a serious impact on environmental pollution. This paper aims to explore the mechanism of how mercury in soil influences the growth of forage, and the effect to grassland caused by high mercury content in soil. Materials and methods In this study, test materials for alfalfa, cover with alkali grass, festuca arundinacea, grass seed were seeds from East Wuzhu Muqinqi grassland station in Inner Mongolia of Chinese Academy of Sciences. Test the soil collected from East Wuzhu Muqinqi grassland station in Inner Mongolia of Chinese Academy of Sciences. Peroxidase （POD） activity of guaiacol method is used to determine. Catalase （CAT） activity using ultraviolet absorption spectrometry. Malondialdehyde （MDA） using glucosinolates barbituric acid colorimetric method. Proline using sulfosalicylic acid method. Soluble protein using coomassie brilliant blue staining method. Soluble sugar content determination by anthrone method. Results Alkali grass and festuca arundinacea seed germination rate gradually reduced with Hg2＋ concentration in the soil, briefly in the early years of alfalfa seed germination, but high concentration of inhibition. In this paper, research has shown that, under the stress of Hg2＋ in soil, Hg2＋ concentration is lower than 1.5 mg·kg-1, alfalfa POD activity is on the rise, when the Hg2＋ concentration is greater than 1.5 mg·kg-1, alfalfa POD activity falling. Cover with alkali grass POD activity as a whole is on the rise. Tall fescue POD activity smoothly. Under the stress of Hg2＋ in soil, Hg2＋ concentration is lower than 2.0 mg·kg-1, the three kinds of forage grass CAT are on the rise, and when the concen

关 键 词：土壤 汞 牧草 生理生化 

分 类 号：X53[环境科学与工程—环境工程]