‘夏日阳光’番茄侧芽离体培养快繁技术  

In vitro Culture Technique for Rapid Propagation of Lateral Bud of Lycopersicon esculentum ‘Xiariyangguang'

作　　者：赖呈纯[1] 潘红[1] 范丽华[1] 谢鸿根[1] 黄贤贵[1] 

机构地区：[1]福建省农业科学院农业工程技术研究所,福州350003

出　　处：《中国农学通报》2016年第34期49-54,共6页Chinese Agricultural Science Bulletin

基　　金：福建省种业创新与产业化工程项目"智能温室种苗工程化生产技术创新与产业化工程"(2014S1477-1);福建省科技重大专项"现代智能设施农业系统关键技术研究与示范"(2014NZ0002-2);福建省农业科学院设施农业专项科研项目"智能温室番茄生长生物学及其农艺操作规程的研究"(FQ1405)

摘　　要：‘夏日阳光’番茄是近年来引进的高端品种,丰产性好、品质高,深受市场的青睐,但其种子为F1代杂种,价格昂贵,很难在市场上大面积推广。利用植物离体培养技术快速繁殖‘夏日阳光’番茄种苗,是生产上推广该品种的一条可行途径。为快速建立‘夏日阳光’番茄无菌株系以及获得其试管芽苗的高效增殖,以‘夏日阳光’番茄侧芽为材料,探讨其离体培养过程中的关键技术。试验结果表明,采用0.1%Hg Cl2溶液(w/v)加2滴土温20消毒8 min的处理方式消毒后,培养在MS+0.1 mg/L BA+0.1 mg/L IBA培养基中,可以较快速地建立‘夏日阳光’番茄无菌株系,成苗率为46.7%;继代增殖适宜的培养基配方为MS+0.5 mg/L BA+0.1 mg/L IBA,增殖系数可达4.3,并且芽苗生长健壮,为其种苗的规模化繁育提供技术基础。Lycopersicon esculentum‘Xiariyangguang’is an imported high-level tomato variety recently,which gets the favor of market because of high yield and high quality. However, it is difficult to be planted inlarge scale for marketing because of its expensive seeds, which are all hybrid F1 generation. Base on the plantin vitro culture techniques, the seedlings of Lycopersicon esculentum‘Xiariyangguang’will be propagatedrapidly. Therefore, it is a feasible way for promoting this variety in production. In order to rapidly establishaseptic lines of Lycopersicon esculentum‘Xiariyangguang’and obtain high-performance proliferation of itsshoots in vitro, the key techniques were investigated by using its lateral buds as materials during its in vitroculture. The results showed that after its explant was disinfected by using 0.1% mercuric chloride with someTween 20 treatment for 8 min, the aseptic lines of Lycopersicon esculentum‘Xiariyangguang’could beestablished in media MS＋ 0.1 mg/L BA＋ 0.1 mg/L IBA, whose seedling rate was 46.7%. The medium MS＋0.5 mg/L BA＋ 0.1 mg/L IBA was suitable for bud seedling subculture and proliferation to obtain healthy andstrong plantlets. Its multiplication rate could reach 4.3. These techniques could lay the foundation for large-scale propagation of Lycopersicon esculentum‘Xiariyangguang’ seedlings.

关 键 词：番茄 侧芽 外植体 增殖 快繁技术 

分 类 号：S641.2[农业科学—蔬菜学] Q813.1[农业科学—园艺学]