新疆核桃树腐烂病致病力室内测定方法的研究  

Laboratory Evaluation Method of Walnut Canker Pathogenicity

作　　者：尹永香[1] 刘应敏[1] 马荣[2] 赵丽[2] 何美茹 蔡桂芳[2] 

机构地区：[1]新疆农业大学林业研究所,乌鲁木齐830052 [2]新疆农业大学林学与园艺学院,乌鲁木齐830052

出　　处：《中国农学通报》2016年第34期108-112,共5页Chinese Agricultural Science Bulletin

基　　金：新疆维吾尔自治区自然科学基金"新疆核桃树腐烂病病原菌致病性分化研究"(2014211B019)

摘　　要：为探索出快速、可靠及操作简便的新疆核桃树腐烂病室内致病力测定方法,通过不同伤口类型将强致病力菌株的菌饼、分生孢子悬浮液和菌悬液接种在‘温185’核桃品种3种枝龄的枝条上,观测4种不同温度处理后核桃树腐烂病的发病程度得出最适致病条件。结果表明:3年生枝龄的核桃腐烂病发病最严重,病斑长度达到8.5 cm;通过烫伤处理后利用菌饼接种的离体枝条病斑长度最大,为9.8 cm;最利于病原菌扩展的温度为25℃,病斑长度达到11 cm;烫伤后放置0、1、2、3、4天后接种,病斑长度扩展无显著性差异性。研究结果为核桃腐烂病室内致病力的快速测定提供理论依据。The purpose of this project was to establish a fast, reliable and easy method to determine the virulence of walnut canker. Mycelium, spore suspension and bacterial suspension were inoculated on 1, 2 and3 years old branches of‘Wen 185’with different wound treatments, and then pathogenic severity was observedwith 4 different temperature treatments and then the optimium disease condition was obtained. The resultsshowed that the canker of 3 years old walnut was most serious and the lesions length was up to 8.5 cm; the mostideal inoculum and wound treatments were agar disk and scalding because the lesions length was up to 9.8 cm;25℃ was the optimum temperature for pathogenic bacteria to grow and the lesions length was up to 11 cm, andthere was no difference of the inoculation time after scalding for the growth of pathogenic bacteria. Theresearch results provide the oretical basis for a rapid laboratory determining method of walnut canker pathogenicity.

关 键 词：核桃腐烂病 致病性测定 伤口类型 接种体 温度 枝龄 

分 类 号：S763.15[农业科学—森林保护学]