黄尾鲴6种同工酶的组织特异性研究  

Six Kinds of Isozymes in Different Tissues of Xenocypris davidi

作　　者：张燕萍[1] 徐先栋[1] 章海鑫[1] 李彩刚[1] 

机构地区：[1]江西省水产科学研究所,南昌330039

出　　处：《中国农学通报》2016年第35期10-14,共5页Chinese Agricultural Science Bulletin

基　　金：江西省科技计划项目"黄尾密鲴良种选育及健康养殖技术研究与推广"(20141BBF60036)

摘　　要：为了给黄尾鯝种质标准和种质鉴定提供生化遗传指标,以便合理保护和利用黄尾鯝种质资源。采用聚丙烯酰胺垂直凝胶电泳法对黄尾鲴的7种新鲜组织(肝脏、心脏、脑、肌肉、脾脏、头肾和鳃)中的苹果酸脱氢酶(MDH)、乳酸脱氢酶(LDH)、酯酶(EST)、苹果酸酶(ME)、6-磷酸葡萄糖脱氢酶(6-PGDH)、超氧化物岐化酶(SOD)共6种同工酶进行了分析,并对其同工酶位点及酶谱进行了分析。结果表明,黄尾鯝的6种同工酶在不同组织中的表达存在差异。酯酶、苹果酸酶、苹果酸脱氢酶及6-磷酸葡萄糖脱氢酶在肝脏中表达活性较强,乳酸脱氢酶在肝、脾以及头肾组织中表达活性强,超氧化物歧化酶在肝、脾脏组织中活性较强。To provide biochemical genetic indexes for germplasm standard and identification of Xenocypris davidi, so as to appropriately protect and utilize the resources, malate dehydrogenase（MDH）, lacticdehydrogenase（LDH）, esterase（EST）, malic enzyme（ME）, 6-phosphogluconate dehydrogenase（6-PDGH） andsuperoxide dismutase（SOD） from seven tissues（liver, heart, brain, muscle, spleen, kidney and gill） of Xenocypris davidi were studied by using vertical gradient polyacrylamide gel electrophoresis. Isozyme loci andzymograms were also analyzed. The results showed that the six isozymes presented tissue specificity to variousdegrees. The activities of EST, ME, MDH and 6-PDGH in liver were high. The activity of LDH was high inliver, spleen and kidney. The activity of SOD was high in spleen and liver.

关 键 词：黄尾鲴 同工酶 组织特异性 

分 类 号：S917.4[农业科学—水产科学]