男性超体质量与精液参数及精浆TNF-α、IL-6水平的相关性  

The correlation between over-weight and semen paraments, and the level of TNF-α, IL-6 in seminal plasma of men

作　　者：樊卫民[1,2] 刘悦[1] 丁之德[1] 

机构地区：[1]上海交通大学医学院解剖学与组织胚胎学系,上海200025 [2]上海交通大学医学院附属瑞金医院生殖医学中心

出　　处：《中国男科学杂志》2016年第8期14-17,22,共5页Chinese Journal of Andrology

摘　　要：目的检测超体质量男性精浆中细胞因子TNF-α、IL-6水平含量,探讨其水平改变与超体质量的相关性及其对精液质量各参数的影响。方法从上海交通大学医学院附属瑞金医院生殖医学中心门诊就诊的男性患者中选取25例为超体质量组;另选取正常健康男性25例为对照组。对两组精液质量进行分析,用ELISA法检测精浆中TNF-α、IL-6水平含量。结果对照组精液体积、前向运动精子及正常形态精子都明显高于超体质量组(P<0.01);对照组精液浓度高于超体质量组,但无统计学意义。对照组精浆IL-6、TNF-α水平明显著低于超体质量组(P<0.01)。精浆IL-6、TNF-α均与体质量指数(BMI)呈正相关性(P<0.01),前向运动精子、精液体积和正常形态精子均与BMI呈负相关(P<0.01),TNF-α、IL-6与正常形态精子之间呈负相关(P<0.05)。结论男性超体质量患者精浆TNF-α、IL-6水平显著增加,其产生的毒性作用可引起精子形态和功能的改变,继而导致男性不育的发生。检测精浆中TNF-α、IL-6水平可为临床分析超体质量或肥胖所导致男性不育的病理机制提供重要的诊断依据。Objective To detect the cytokine TNF-α, IL-6 levels in seminal plasma of over-weight male, and explore the correlation between the levels of TNF-α, IL-6 and over-weight, as well as its effects on semen paraments. Methods Both of 25 males with overweight and 25 healthy males were enrolled in the study from The Center of Reproductive Medicine, Ruijing Hospital, Shanghai Jiao Tong University School of Medicine. We analyzed the semen parameter from the two different groups and detected the levels of TNF-α and IL-6 in seminal plasma. Results Compared with those of over-weight group, levels of semen volume, sperm mobility of class A, sperm activity and total sperm viability were all significantly increased in the control group（P〈0.01）. The concentration of sperm in the control group was higher than that in over-weight group, but there was no statistical significance. The levels of IL-6 and TNF-α in the control group were lower than that in over-weight group（P 〈0.01） and there was a positive correlations between IL-6, TNF-α levels and overweight（P 〈0.01）, whereas the correlations remained negative between sperm mobility of type A, semen volume, normal form sperm and over-weight（P 〈0.01）. Moreover, there was a negative correlation between the levels of IL-6,TNF-α in seminal plasma and the normal form sperm（P 〈0.05）. Conclusion Over-weight male usually had a high levels of TNF-α and IL-6 in the seminal plasma. However, the high levels of these molecules maybe influence sperm development and maturation by impairing the quality of sperm and altering sperm morphology. This study may provide an important pathogenic cue for the male infertility with overweight or obesity.

关 键 词：超体质量 精液 肿瘤坏死因子Α 白细胞介素6 不育 男性 

分 类 号：R698.2[医药卫生—泌尿科学]