重铬酸钾对泥鳅鳍细胞系的毒理学研究  

Cytotoxicity of potassium dichromate to loach fin cell lines in vitro

作　　者：吴迪[1] 周诗珈 李霞[1,2] 秦艳杰[1] 白丽雯[1] 王文文[1] WU Di ZHOU Shi-jia LI Xia QIN Yan -jie BAI Li-w e n WANG Wen -wen(Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning Province,Dalian Ocean Lniversity,Dalian 116023,China Key Laboratory of Mariculture ＆ Stock Enhancement in North China’s Sea,Ministry of Agriculture,Dalian Ocean Lniversity,Dalian 116023, China)

机构地区：[1]大连海洋大学辽宁省海洋生物资源恢复与生境修复重点实验室,辽宁大连116023 [2]大连海洋大学农业部北方海水增养殖重点实验室,辽宁大连116023

出　　处：《大连海洋大学学报》2016年第6期646-650,共5页Journal of Dalian Ocean University

基　　金：国家自然科学基金资助项目(31272650)

摘　　要：为探究重铬酸钾的毒性机制,建立适合监测铬污染的体外检测系统,以体外培养的泥鳅Misgurnus anguillicaudatus鳍细胞系（DIMF）为试验材料,研究了重铬酸钾的毒性效应。结果表明：通过噻唑蓝（MTT）比色法测定重铬酸钾染毒后细胞的24 h半致死浓度为（25.3±1.2）滋mol/L;暴露在浓度为0~30滋mol/L的重铬酸钾中,细胞超氧化物歧化酶（SOD）活性随着染毒浓度的增加而增大;谷胱甘肽超氧化物酶（GSH-Px）在重铬酸钾浓度为0~20滋mol/L时活性升高,当染毒浓度为30 mol/L时,活力开始下降;谷胱甘肽S-转移酶（GST）活性则随重铬酸钾浓度的升高而降低;微核试验显示,DIMF微核率随染毒浓度的增加呈先升高后降低的趋势,其中最大微核率为0.733%;实时定量PCR结果显示,对照组的金属硫蛋白（MT）基因表达量很低,经重铬酸钾诱导后MT基因表达量显著升高（P〈0.01）。研究表明,重铬酸钾可对细胞的酶系统和遗传物质造成一定的损伤。Loach Misgurnus anguillicaudatus fin cell line （ D I M F ） was used to assess toxicity of potassium dichro-mate in vitro to study toxicity mechanism of potassium dichromate and establish suitable for chrome pollution detec-tion system. The results showed that the median lethal concentration （ LC50） was （25. 3 ± 1 . 2 ）μmo l / L in D I M F exposed to potassium dichromate for 24 h measured by thiazole blue （ M TT ） method. T he activities of superoxide dismutase （ SOD ） in DIMF were increased with the increase in concentration within potassium dichromate concen-tration of 0-30 滋 mo^L and the activities of glutathione peroxidase （ G S H -P x ） were increased at 0-20 μmol/ L po-tassium dichromate, and then decreased at concentration of 30 μmol/L,with gradual decrease in glutathione S transferase（ GST ） activity. Micronucleus test revealed that the rate of micronucleus was increased first and then de-creased with the increase in potassium dichromate concentration,with the m a x i m u m micronucleus rate of 0.733%. Real-time PCR showed that metallothionein （MT ） gene expression quantity was very low in control group,and in-creased significantly due to the induction by potassium dichromate（ P〈0. 01）. T he findings indicate that potassium bichromate can damage enzyme system and genetic materials in cells.

关 键 词：泥鳅 重铬酸钾 细胞系 酶活力 微核率 金属硫蛋白 

分 类 号：S917.4[农业科学—水产科学]