结核分枝杆菌IgG抗体蛋白芯片对活动性与非活动性结核患者疗效的评价  

Evaluation of mycobacterium tuberculosis IgG antibody protein chip in patients with active and inactive tuberculosis

作　　者：宋能 刘焰[2] 段元山 马杰 SONG Neng LIU Yan DUAN Yuan-shan MA Jie(Clinical Laboratory, Hubei Provincial Hospital of Integrated Chinese and Western Medicine, Wuhan 430015, China Department of Immunology, Wuhan Universi ty School of Basic Medical Sciences, Wuhan 430015, China)

机构地区：[1]湖北省中西医结合医院检验科,湖北武汉430015 [2]武汉大学基础医学院免疫学系,湖北武汉430071

出　　处：《中国生化药物杂志》2016年第12期174-176,共3页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的探讨结核分枝杆菌IgG抗体蛋白芯片用于活动性结核患者与非活动性结核患者的诊断价值。方法采用结核分枝杆菌IgG抗体蛋白芯片分别对湖北省中西医结合医院检验科178例活动性结核病患者和79例非活动性结核病患者以及92例健康对照血清样本进行检测。结果结核分枝杆菌IgG抗体蛋白芯片对结核病总阳性率为58.4%;3项蛋白的联合诊的辅助诊断价值高于单项蛋白的辅助诊断价值;16k D蛋白对非活动性结核阳性率为16.4%,优于其对活动性结核3.4%的阳性率(P<0.05)。结论结核分枝杆菌IgG抗体蛋白芯片对活动性结核和非活动性结核均具有一定的辅助诊断价值,在非活动性结核患者中16k D蛋白阳性率略高于38k D蛋白(P<0.05)。Objective To explore protein microarray chip diagnostic value for patients with active and inactive tuberculosis. Methods 178 cases of active patients tuberculosis and 79 cases of inactive tuberculosis patients and 92 cases of healthy control using protein microarray chip detection. Results Tuberculosis protein chip had a diagnostic value for tuberculosis and the positive rate is 58. 4%; combined the diagnostic value of three kinds of proteins is higher than the diagnostic value of a single protein; 16 k D protein of inactive tuberculosis positive rate was 16. 4%,better than the positive rate of3. 4% for active tuberculosis（ P〈0. 05）. Conclusion Tuberculosis protein chip has a diagnostic value for active tuberculosis and inactive tuberculosis.16 k D protein positive rate more than 38 k D protein in patients with inactive tuberculosis（ P〈0. 05）.

关 键 词：结核分枝杆菌 蛋白芯片 非活动性结核患者 结核诊断 

分 类 号：R378.911[医药卫生—病原生物学] R825.2[医药卫生—基础医学]