熊果酸对肝细胞胆固醇代谢的影响  

Effects of Ursolic Acid on Cholesterol Metabolism in Hepatic Cells

作　　者：梁奎英[1] 初霞[2] 

机构地区：[1]哈尔滨工程大学医院内科,哈尔滨150001 [2]哈尔滨医科大学公共卫生学院营养与食品卫生学教研室,哈尔滨150081

出　　处：《医药导报》2017年第1期9-12,共4页Herald of Medicine

基　　金：国家自然科学基金资助项目(81202191);黑龙江省普通高等学校营养与食品卫生学重点实验室开放课题(YYKFKT1209)

摘　　要：目的探讨熊果酸对人肝癌细胞Hep G2及小鼠肝细胞AML^(-1)2胆固醇代谢的影响及其相关机制。方法0,10,20,40μmol·L^(-1)熊果酸处理Hep G2及AML^(-1)2细胞24 h,应用反转录-聚合酶链反应(RT-PCR)、Western blotting检测细胞内胆固醇代谢关键基因胆固醇7A-羟化酶(CYP7A1)mRNA和蛋白表达,应用酶法检测细胞内胆固醇浓度。结果与0μmol·L^(-1)熊果酸组比较,20和40μmol·L^(-1)熊果酸组Hep G2及AML^(-1)2细胞内CYP7A1 mRNA及蛋白表达水平均显著增加(均P<0.05),相应的细胞内胆固醇浓度也随之下降(均P<0.05)。结论一定浓度的熊果酸能够降低肝细胞内胆固醇浓度,CYP7A1可能参与了调节过程。Objective To explore the effects and mechanism of ursolic acid（ UA） on cholesterol metabolism in human hepatocellular carcinoma HepG2 and mouse hepatocyte AML-12. Methods HepG2 and AML-12 cells were treated with different concentrations of UA（ 0,10,20,40 μmol·L^-1） for 24 h,then the mRNA and protein expression of cholesterol 7alphahydroxylase（ CYP7A1） and intracellular cholesterol level was detected by RT-PCR、Western blotting and enzymatic method,respectively. Results Compared with 0 μmol·L^-1UA,20 μmol·L^-1and 40 μmol·L^-1UA significantly increased the expressions of CYP7A1 mRNA and protein（ P〈0.05）,and decreased intracellular cholesterol level in HepG2 and AML-12 cells（ P〈0.05）. Conclusion A certain concentration of UA can reduce the level of cholesterol in HepG2 and AML-12 cells.CYP7A1 may be involved in the regulation process.

关 键 词：熊果酸 胆固醇代谢 胆固醇7A-羟化酶 

分 类 号：R282.71[医药卫生—中药学] R965[医药卫生—中医学]