二苯乙烯苷对载脂蛋白E基因敲除小鼠动脉粥样硬化的防治作用  

Prevention of 2,3,5,4'-Tetrahydroxystilbene-2-O-beta-D-Glucoside on Atherosclerotic Plaque Formation in Apo E Knock-out Mice

作　　者：张又枝[1,2] 黄琦[2] 闵清[2] 廖清船[2] 蔡飞[1] 

机构地区：[1]湖北科技学院糖尿病心脑血管病变湖北省重点实验室,咸宁437100 [2]湖北科技学院药学院药理教研室,咸宁437100

出　　处：《医药导报》2017年第1期13-16,共4页Herald of Medicine

基　　金：湖北科技学院糖尿病专项(ZX1308);湖北科技学院博士启动基金资助项目(BK1416);湖北省自然科学基金资助项目(2014CFC1080);湖北省教育厅基金资助项目(D20142802)

摘　　要：目的探讨二苯乙烯苷对载脂蛋白E(Apo E)敲除小鼠动脉粥样硬化的防治作用及其机制。方法 Apo E敲除小鼠24只,随机分为正常对照组、模型对照组(高脂饲料)和治疗组(二苯乙烯苷20 mg·kg-1灌胃给药,每天1次),每组8只。通过主动脉和心脏主动脉窦冰冻切片油红O染色,观察二苯乙烯苷对Apo E基因敲除小鼠动脉粥样硬化斑块的作用;Western blotting方法检测二苯乙烯苷对C反应蛋白(CRP)诱导的人脐静脉内皮细胞血凝素样氧化低密度脂蛋白受体1(LOX-1)蛋白表达的影响。结果正常对照组主动脉未见明显的斑块沉积,模型对照组呈现红色着色和点状脂质条纹沉积;与模型对照组比较,治疗组血管脂质沉积减少,心脏主动脉窦粥样斑块面积减小。二苯乙烯苷可降低CRP诱导的LOX-1蛋白表达。结论二苯乙烯苷可能通过抑制LOX-1的蛋白表达,减少Apo E基因敲除小鼠动脉粥样硬化斑块形成。Objective To observe the protective effect and mechanism of 2,3,5,4 ＇-tetrahydroxystilbene-2-O-beta-Dglucoside（ THSG） on atherosclerosis in ApoE konck-out mice. Methods A total of 24 ApoE knock-out mice were randomly divided into normal control group（ n = 8）,model control group（ HFD,high-fat diet,n = 8） and treated group（ THSG,20 mg·kg^-1,i. g.,n = 8）. The atherosclerosic plaque of aorta wall and aorta root were measured by oil red O staining; The expression of lectin-like oxidized low-density lipoprotein receptor-1（ LOX-1） in human umbilical vein endothelial cells（ HUVEC） through C-reaction protein（ CRP） was studied by Western blotting. Results The atherosclerosis plaque in normal control group was not observed. The lipid accumulation decreased in the aorta and the plaque areas in the aortic sinus in THSG treated-group compared with model control group. Moreover,THSG down-regulated CRP-induced LOX-1 expression in HUVEC. Conclusion The atheroscletosis plaque in ApoE knock-out mice was decreased by THSG. The mechanism might be related to the inhibition of the expression of LOX-1 protein.

关 键 词：二苯乙烯苷 血凝素样氧化低密度脂蛋白受体1 载脂蛋白E基因敲除 动脉粥样硬化 

分 类 号：R282.71[医药卫生—中药学] R965[医药卫生—中医学]