一株产氢菌的分离鉴定与产氢特性  

Isolation and characterization of a hydrogen-production bacteria

作　　者：张安龙[1] 董婷婷[1] 王雪青[1] 王晔[1] ZHANG An-long DONG Ting-ting WANG Xue-qing WANG Ye(College of Bioresources Chemical and Materials Engineering, Shaanxi University of Science Technology, Xi’an 710021, China)

机构地区：[1]陕西科技大学轻工科学与工程学院,陕西西安710021

出　　处：《陕西科技大学学报（自然科学版）》2017年第1期40-44,共5页Journal of Shaanxi University of Science & Technology

基　　金：国家十二五科技支撑计划项目(2011BAC11B04);陕西科技大学研究生创新基金项目

摘　　要：从造纸厂厌氧颗粒污泥中分离出一株高效的产氢细菌DW01,通过16SrDNA序列分析,表明DW01菌株属于Raoultella属,与Raoultella sp.NGB-FR77相似性为100%.同时,在温度为30℃的条件下,进一步优化了DW01菌株在不同碳源、氮源、pH的培养条件下发酵产氢性能,结果表明,该菌株在以葡萄糖为碳源,L-谷氨酸为氮源,初始pH为6.0的条件下,获得最佳氢气产量和最佳产氢速率,分别为188.9±2.1mL/g和93.3±7.5mL/L/h.DW01菌株作为良好的发酵产氢菌,可进一步优化产氢条件,提高产氢量.An efficient hydrogen-producing bacteria named DW01 was isolated from the anae-robic paper mill granular sludge, and the 16S rDNA sequence analysis showed that DW01 strain belonged to Raoultella genus,and was identified having high similarity of 100% with Raoultella sp. NGB-FR77. Meanwhile, under temperature of 30℃ conditions, effects of car-bon sources,nitrogen source and initial pH on the hydrogen production with DW01 were also studied. Results showed that when the strain utilized glucose as the carbon source, L-glutamic acid as nitrogen source under initial pH6. 0 condition, the maximum hydrogen yield and hydrogen production rates were obtained as 188. 9±2. 1 mL/g and 93. 3zb7. 5 mL/L/h. For DW01 strain was regarded as a advantage hydrogen production bacteria,it should be further optimized to improve the hydrogen production performance.

关 键 词：生物制氢 厌氧污泥 分离鉴定 产氢优化 

分 类 号：Q939[生物学—微生物学]