Th17细胞在特发性血小板减少性紫癜发病中的作用和意义  

Role and Significance of T Help Cells 17 in Pathogenesis of Idiopathic Thrombocytopenic Purpura

作　　者：王潇[1] 周郁鸿[2] 陈小红[1] 尹利明[1] 赵燕娜[1] 沃立科[1] 

机构地区：[1]浙江省中医院血液病研究所,浙江杭州310006 [2]浙江省中医院血液科,浙江杭州310006

出　　处：《中国实验血液学杂志》2016年第6期1833-1836,共4页Journal of Experimental Hematology

摘　　要：目的:本研究探讨Th17细胞在特发性血小板减少性紫癜(ITP)发病中的作用和意义。方法:采用FCM检测ITP患者组和正常对照组外周血中Th17细胞的比例;ELISA技术检测上述两组血浆中IL-17、IL-23、IL-6和TGF-β1的表达水平;RT-PCR技术检测外周血单个核细胞(PBMNC)中IL-17和RORγt mRNA的表达水平;Westernblot法检测PBMNC中pSTAT3和RORγt蛋白的表达水平。结果:ITP患者外周血Th17细胞比例较正常对照组显著升高,血浆中IL-17、IL-23、IL-6和TGF-β1水平明显高于正常对照组(P<0.01);ITP患者PBMNC中IL-17和RORγt mRNA表达水平明显高于正常对照组(P<0.05);p STAT3和RORγt蛋白的表达水平明显高于正常对照组(P<0.05))。结论:在ITP发生和发展中,Thl7细胞亚群比例增高可能是一个重要的决定因素,Th17细胞相关的细胞因子及转录调控因子水平的变化与ITP发病密切相关。Objective：To investigate the role and significance of T help cells 17（Th17） in pathogenesis of idiopathic thrombocytopenic purpura（ITP）.Methods：Peripheral blood samples from ITP patients and normal controls were examined for Th17 cell proportion by flow cytometry（FCM）.Expression of IL-17,IL-23,IL-6 and TGF-β1 in hematoplasma was detected by ELISA.The mRNA expression level of IL-L7 and RORγt in peripheral blood mononuclear cells（PBMNC） from patients with ITP and normal controls were measured by RT-PCR technique,and expression levels of pSTAT3 and RORγt proteins were analyzed by Western-blot.Results：Th17 cells in peripheral blood from patients with ITP was greatly increased when compared with normal control group（P〈0.05）.Expressions of IL-17,IL-23,IL-6 and TGF-β1 in hematoplasma of ITP patients were all significantly higher than those in normal control group（all P 〈0.01）.mRNA expression levels of IL47 and RORγt in PBMNC from patients with ITP were much higher than those in normal controls（P〈0.05）.Protein expressions of pSTAT3 and RORγt in PBMNC of ITP patients were greatly increased as compared with those in control（P〈0.05）.Conclusion：Th17 cell subgroup may play a role in incidence and development of ITP,which may participate in the pathogenesis of ITP by increasing Th17 cell proportion and altering the expression level of Th17-related cytokines as well as regulatory and transcriptional factors.

关 键 词：特发性血小板减少性紫癜 TH17细胞 白介素-17 

分 类 号：R558.2[医药卫生—血液循环系统疾病]