不同贮存时间的单采少白细胞血小板中精氨酸酶水平的研究  

Arginase Level in Apheresis Leukocyte-Reduced Platelets Stored for Different Time

作　　者：黄豪博[1] 范丽萍[1] 魏世金[1] 傅丹晖[1,2] 曾丰[1] 黄清华[1] 

机构地区：[1]福建医科大学附属协和医院输血科,福建福州350001 [2]福建医科大学附属协和医院血液科、福建省血液病研究所,福建福州350001

出　　处：《中国实验血液学杂志》2016年第6期1879-1882,共4页Journal of Experimental Hematology

基　　金：福建省中青年教师教育科研项目(JA14133);2015年福建省卫生系统中青年骨干人才培养项目基础项目(2015-ZQN-JC-18);2016年福建省自然科学基金计划项目(2016J01461)

摘　　要：目的:探讨不同贮存时间对单采少白细胞血小板(apheresis leukocyte-reduced platelet,ALR-Plt)中精氨酸酶水平的影响及精氨酸酶的可能来源。方法:应用ELISA法检测不同贮存时间ALR-Plt和对照血浆中精氨酸酶水平及髓过氧化物酶(myeloperoxidase,MPO)水平;应用相关性分析分析不同贮存时间的ALR-Plt中精氨酸酶水平与MPO水平的相关性。结果:贮存3 d内的ALR-Plt与对照血浆中精氨酸酶水平差异无统计学意义,但是贮存超过3d的ALR-Plt中精氨酸酶水平显著高于贮存3 d内的ALR-Plt和对照血浆中的水平(P<0.05)。不同贮存时间的ALR-Plt之间MPO水平差异无统计学意义,但均显著低于对照血浆中的水平(P<0.05)。相关性分析显示:ALRPlt中精氨酸酶水平与MPO水平呈正相关(r=0.58)。结论:贮存时间超过3 d的ALR-Plt中精氨酸酶水平显著升高,其可能来源于残留的少量白细胞,特别是中性粒细胞。Objective：To explore the effect of storage time on arginase level and possible source of arginase in apheresis leukocyte-reduced platelets（ALR-P1t）.Methods：The arginase level and myeloperoxidase（MPO） levels in ALR-Plt and control plasma were detected by ELISA.The relationship between arginase level and MPO level in ALR-Plt was analyzed by correlation analysis.Results：There was no significant difference of arginase level between ALR-Plt stored less than 3 days and control plasma.However,arginase level in ALR-Plt stored over 3 days was significantly higher than that in ALR-Plt stored less than 3 days and control plasma（P〈0.05）.There was no significant difference of MPO level in ALR-Plt stored for different times,but the MPO level in ALR-Plt stored for different time was lower than that in control plasma.Correlation analysis showed that arginase level positively correlated with MPO level in ALR-Plt of different storage time（r =0.58）.Conclusion：The arginase level in ALR-Plt stored over 3 days increase significantly.The main possible source of arginase in ALR-Plt is the residual white blood cells,especially neutrophils.

关 键 词：单采少白细胞血小板 贮存时间 精氨酸酶 髓过氧化物酶 

分 类 号：R457.1[医药卫生—治疗学] R331.143[医药卫生—临床医学]