胚胎干细胞来源神经干细胞调控巨噬细胞的实验研究  

Effect of embryonic stem cells-derived neural stem cells on macrophage in vitro

作　　者：祝文 袁亚迎 焦峰军 ZHU Wen YUAN Ya-Ying JIAO Feng-Jun(Department of Critical Care Medicine, the First People＇s Hospital of Xianyang City ,Xianyang 712001, China)

机构地区：[1]咸阳市第一人民医院重症医学科,咸阳712001

出　　处：《中国免疫学杂志》2016年第12期1741-1744,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金(81571209)资助项目

摘　　要：目的:观察胚胎干细胞(Embryonic stem cell,ESC)来源的神经干细胞(Neural stem cells,NSCs)对骨髓巨噬细胞的增殖、吞噬及分泌细胞因子的影响,为探讨NSCs免疫调节及在重大疾病中的应用做铺垫。方法:培养骨髓巨噬细胞,收集胚胎干细胞来源NSCs(ESC-NSCs)的上清液处理巨噬细胞,然后利用磺酰罗丹明B(Sulforhodamine B,SRB)法检测增殖情况;利用红荧光beads检测吞噬能力;ELISA法检各组TNF-α和IL-1β表达情况。结果:成功从ESC获得具有NSCs特征的细胞。对照组、NSC组巨噬细胞增殖率分别为100%、(126.29±5.41)%,beads吞噬率分别为(70.23±2.57)%、(90.32±8.49)%。相比对照组,NSC组巨噬细胞TNF-α和IL-1β含量下降(P<0.05)。结论:ESC来源的NSCs具有促进骨髓巨噬细胞增殖及增强其吞噬能力,并抑制其炎性因子分泌,为NSCs免疫调节作用提供新的依据。Objective： To explore the influence of embryonic stem cells-derived neural stem cells on the proliferation and secretion cytokines of bone marrow-derived macrophages. Methods： Mouse bone marrow derived macrophages were isolated and cultured in L929 medium. After macrophages were treated with NSCs supernatant for 3 days,SRB method was used to detect the proliferation of macrophages. The phagocytosis of macrophages were detected by incubating with RFP-Beads for 1 h. Meanwhile,the expression of TNF-α and IL-1β were detected by ELISA. Results： NSCs were successfully induced from ESC. In control group and NSC group,the proliferation rate of macrophages were 100 % and（ 126. 29 ± 5. 41） %,the phagocytosis rate were（ 70. 23 ± 2. 57） % and（ 90. 32 ±8. 49） %. Compared to the control group,the levels of IL-6,IL-1β in macrophage treated with NSCs decreased（ P〈0. 05）. Conclusion：ESC-derived NSCs can promote the proliferation and phagocytosis of bone marrow-derived macrophage,and suppress the secretion of pro-inflammatory cytokines.

关 键 词：神经干细胞 胚胎干细胞 巨噬细胞 吞噬 细胞因子 

分 类 号：R392.4[医药卫生—免疫学]