BALB/c小鼠免疫重组蓖麻毒素B链蛋白的免疫机制研究  

Study on immune mechanism of a recombinant ricin B chain protein against ricin intoxication in BALB/c mice

作　　者：王俊虹[1] 吴绘[1] 魏茂提 WANG Jun-Hong WU Hui WEI Mao-Ti(Logistics University of PAP, Tianjin 300309, Chin)

机构地区：[1]武警后勤学院,天津300309 [2]天津市职业与环境危害防制重点实验室,天津300309

出　　处：《中国免疫学杂志》2016年第12期1758-1760,共3页Chinese Journal of Immunology

基　　金：武警后勤学院开放基金项目(WHKF201503);军事预防医学"2110"三期工程建设项目资助

摘　　要：目的：探讨重组蓖麻毒素B链蛋白（rRTB）免疫雌性BALB/c小鼠的免疫机制。方法：雌性BALB/c小鼠分为rRTB组和PBS组,间隔14 d皮下多点注射,共4次。间接ELISA分析小鼠血清抗体效价和抗体分型,采用流式细胞仪检测脾细胞因子IFN-γ和IL-4。结果：血清IgG效价达到1∶10-7以上,且攻毒后产生二次免疫应答,与PBS对照组差别有统计学意义（P〈0.05）;IgG1达到1∶10-5以上,与PBS对照组差别有统计学意义（P〈0.05）;Ig G2a未发生明显变化。细胞因子检测结果表明IFN-γ的表达量与PBS组差异无统计学意义,而IL-4的表达量显著提高（P〈0.05）。结论：rRTB蛋白免疫可刺激小鼠产生高水平的特异性抗体和细胞因子,诱导以IgG1为主的抗体和Th2优势应答,为候选疫苗的研制奠定基础。Objective： To explore the immunity provided to BALB/c mice by immunization with the recombinant ricin B chain protein（ rRTB）. Methods： Female BALB / c mice were randomly selected into rRTB-vaccinated group and PBS group. Mice were subcutaneously（ s. c.） injected four times with 14 days immunization time interval. Changes of antibodies（ Ig G,Ig G1 and Ig G2a） in serum were detected by ELISA. Meanwhile,the expression of IL-4 and IFN-γ were detected by flow cytometry. Results： The mean Ig G titers reached 106 after the fourth immunization and a strong secondary response was induced in vaccinated mice when challenged with toxin. There was significant difference between rRTB-vaccinated group and PBS group（ P〈0. 05）. The same result was shown in Ig G1. However,no changed was detected in Ig G_（2a）. Meanwhile,there was significant difference for IL-4 between two groups（ P〈0. 05）,while no significant difference for IFN-γ was observed. Conclusion： rRTB can produce higher levels of antigen-specific antibodies（ Ig G and Ig G_1）,and cytokine（ IL-4） of splenocytes,which means the recombinant protein can induce the Th2-type immune response and trigger a good immune response. rRTB may be a potentially valuable vaccine candidate against human exposure to AT.

关 键 词：蓖麻毒素 B链蛋白 免疫应答 生物恐怖 

分 类 号：R392[医药卫生—免疫学]