杜鹃兰组培快繁基本条件研究  

Study on the Basic Conditions of Tissue Culture and Rapid Propagation of the Cremastra appendiculata( D. Don) Makino

作　　者：彭斯文 朱校奇 黄艳宁 高晓峰[2] 张明生[2] Peng Siwen Zhu Xiaoqi Huang Yanning Gao Xiaofeng Zhang Mingsheng(Agricultural and Biological Resources Utilization Institute of Hunan Academy of Agricultural Sciences, Changsha, 410125, China College of Life Sciences, Guizhou University, Guiyang 550025, China)

机构地区：[1]湖南省农业生物资源利用研究所,湖南长沙410125 [2]贵州大学生命科学学院,贵州贵阳550025

出　　处：《中国野生植物资源》2016年第6期21-23,26,共4页Chinese Wild Plant Resources

基　　金：国家自然科学基金项目(81360613);湖南省农科院创新培育团队项目(2014TD09)

摘　　要：采用植物组培快繁生物技术,探索p H值、蔗糖浓度、植物生长调节剂类型和浓度等对杜鹃兰组培快繁的影响;运用正交试验方法筛选杜鹃兰组培快繁适宜条件和培养配方。结果表明：杜鹃兰组培的最佳条件是p H值5~6、蔗糖浓度为3%;增殖培养选用激素IAA,分化培养选用激素NAA。最佳培养方案为：光照时间为12 h/d、温度为25℃、6-BA浓度1.5 mg/L、IAA浓度0.75 mg/L。Growth effects of Cremastra appendiculata tissue culture and rapid propagation of the pH value, the concentration of sucrose and auxin type was explored by biological technology. The best conditions and formu- lation were screened by orthogonal experiment method. The results showed that the optimum conditions for the culture of the Cremastra appendiculata were pH 5 -6 and sucrose concentration of 3%, proliferation culture selection IAA, differentiation culture use NAA. The best culture programme is： The illumination time was 12 h/d, the temperature was 25, 6 - BA concentration was 1.5 mg/L, and IAA concentration was 0.75 mg/L.

关 键 词：杜鹃兰 组培快繁 增殖倍数 

分 类 号：Q813.1[生物学—生物工程]