痹痛灵胶囊中人参皂苷Rg1、Rb1及三七皂苷R1的含量  

Determination of ginsenoside Rg1 and Rb1,notoginsenoside R1 in Tong Biling Capsule by HPLC

作　　者：强选萍[1] 顿宝生[1] 杜志德 张建平[1] Xuanping Qiang Baosheng Dun Zhide Du(Shaanxi institute of international trade ＆ Commerce Xianyang 712046)

机构地区：[1]陕西国际商贸学院,陕西咸阳712046

出　　处：《内蒙古中医药》2016年第15期148-149,共2页Inner Mongolia Journal of Traditional Chinese Medicine

基　　金：陕酉省科技厅社会攻关项目:课题名称:基于"化瘀除痹"的痹痛灵治疗AR;RA的临床及实验研究编号2011K16-07-08;陕西省教育厅专项目:课题名称:改进地藏外用药"抗风痛宁"工艺及临床研究编号09JK403;咸阳市科技局发展计划中药现代化项目:课题名称:基于"化瘀除痹"的痹痛灵治疗AR;RA的临床前研究编号2010K14-02;咸阳市科技局发展计划中药现代化项目:课题名称:对古方"抗风痛宁"治疗AR;RA的工艺及临床研究编号09XK12-10

摘　　要：目的：采用高效液相色谱法（HPLC）测定痹痛灵胶囊中人参皂苷Rg1、Rb1及三七皂苷R1的含量。方法：色谱条件：以十八烷基硅烷键合硅胶为填充剂，以乙腈为流动相A，以水为流动相B，柱压11．0mPa，流速：1．0mL／min，柱温：45℃，检测波长为203nm，理论板数按三七皂苷R1峰计算应不低于4000。拖尾因子为1．03。结果：本测定法精密度、重复性、稳定性、回收率良好。人参皂苷Rgl进样量在0．8016—8．0160μg，Rb1在0．8320—8．3200μg，三七皂苷R1在0．2000—2．0000μg内皆与峰面积呈良好的线性关系。结论：本法简便、灵敏、准确、精密度、重复性、稳定性、回收率良好，可有效的控制痹痛灵胶囊的质量。Objective： To determine the content of ginsenoside Rgl, Rb1 and 37 saponins R1 in Bi Tong Ling Capsule by high performance liquid ehro- matngraphy （HPLC）. Methods： The chromatographic conditions： Eighteen alkyl silane bonded silica as filler, with acetonitrile as mobile phase A, with water as mobile phase B, pressure 11. 0mPa, flow rate： 1. 0mL/min, column temperature： 45 degrees centigrade, the detection wavelength was 203nm, the number of theoretical plates should not be less than 4000 calculated by 37 sapenins R1 peak. The tailing factor is 1. 03. Results： The precision, re- peatability, stability and recovery of the method were good. Ginsenoside Rgl injection in 0. 8016 ~ 8. 0160g, Rb1 in 0. 8320 ~ 8. 3200 μg, 37 saponin RI in 0. 2000 ~ 2 μg in the peak area is good linear relationship. Conclusion： This method is simple, sensitive, accurate, precise, reproducible, stable and good recovery rate. It can effectively control the quality of the capsule.

关 键 词：痹痛灵胶囊 高效液相色谱法 人参皂苷Rg1、Rb1 三七皂苷R1 

分 类 号：R473.71[医药卫生—护理学]