周期性牵拉与TNF-α对角膜成纤维细胞增殖的影响  

Effects of Cyclic Stretch and TNF-α on Cell Proliferation of Corneal Fibroblasts

作　　者：姚艳[1] 冯鹏飞[2] 李晓娜[2] 贺瑞[3] 陈维毅[2] 王晓君[1] YAO Yan FENG Pengfei LI Xiaona HE Rui CHEN Weiyi WANG Xiaojun(College of Mechanics Institute of Applied Mechanics and Biomedical Engineering, Taiyuan University of Technology, Tai yuan 030024, China Excimer Laser Department, Shanxi Eye Hospital, Tai yuan 030002, China)

机构地区：[1]太原理工大学力学学院,太原030024 [2]太原理工大学应用力学与生物医学工程研究所,太原030024 [3]山西省眼科医院准分子激光室,太原030002

出　　处：《太原理工大学学报》2016年第1期108-112,共5页Journal of Taiyuan University of Technology

基　　金：国家自然科学基金资助项目:在体角膜材料生物力学特性研究及基于角膜微结构的建模分析(11402161);非糖基化交联后基质刚度变化对角膜基质重塑的调控机理研究(11402162);山西省高等学校教学改革资助项目(J2012014)

摘　　要：利用Flexcell4000柔性基底拉伸系统对角膜成纤维细胞实施应变为5%或15%、频率为0.1Hz的周期性牵拉载荷,用质量浓度为1ng/mL或10ng/mL的肿瘤坏死因子α(Tumor necrosis factor-α,TNF-α)处理,并采用流式细胞仪检测细胞周期,研究周期性牵拉与TNF-α对角膜成纤维细胞增殖的影响。结果表明:5%周期性牵拉或TNF-α单独作用对正常及圆锥角膜成纤维细胞S期细胞比例均无显著性影响;15%牵拉使正常及圆锥角膜成纤维细胞S期细胞比例均显著下降;与正常角膜相比,15%牵拉和TNF-α共同作用使圆锥角膜S期细胞比例下降更显著。周期性牵拉及TNF-α可能通过抑制圆锥角膜基质细胞增殖参与圆锥角膜的发生和发展。Corneal fibroblasts isolated from normal corneas and keratoconus in vitro were sub-jected to cyclic stretch （0. 1 Hz, 5% or 15% strain） using Flexcell 4000 tension system, in thepresence of 1 ng/mL or 10 ng/mL tumor necrosis factor-a （TNF-a）. Cell cycle was detected byflow cytometry to study the effect of cyclic stretch and TNF-a on proliferation of fibroblasts inkeratoconus corneas. The experiment result shows that, 5% strain orTNF-a had no significanteffects on S phase proportion of keratoconus and normal corneal fibroblasts. All groups S phaseproportions of keratoconus and normal corneal fibroblasts decreased under 15% stretch. Com-pared with normal corneal fibroblasts, keratoconus fibroblasts decreased in S phase proportionmore significantly under the effect of 15% stretch and TNF-a,and there is no difference betweenkeratoconus groups. By this study,we know that cyclic stretch and TNF-a may promote the de-velopment of keratoconus by inhibiting the proliferation of corneal stromal cells.

关 键 词：周期性牵拉 肿瘤坏死因子 圆锥角膜 成纤维细胞 细胞增殖 

分 类 号：R318.01[医药卫生—生物医学工程]