饲料维生素C含量对圆斑星鲽幼鱼抗氨氮胁迫能力的影响  

Effects of Dietary Vitamin C Content on Anti-Ammonia-Nitrite Stress Ability of Juvenile Spotted Halibut(Verasper variegatus)

作　　者：王贞杰[1,2] 叶保民 常青[1] 陈四清[1] 刘长琳[1] 胡建成[1] 严俊丽[1,2] 卢斌[1,2] 

机构地区：[1]农业部海洋渔业资源可持续利用重点开放试验室,中国水产科学研究院黄海水产研究所,青岛266071 [2]上海海洋大学水产与生命学院,上海201306 [3]辽宁省海洋与渔业厅,沈阳110000

出　　处：《动物营养学报》2016年第12期4054-4062,共9页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：中国水产科学研究院黄海水产研究所基本科研业务费资助(20603022016005)

摘　　要：氨氮污染是水产养殖中重要的污染物,因此提高鱼体抗污染应激能力至关重要。本试验采用不同维生素C含量的饲料饲喂圆斑星鲽幼鱼,探究饲料维生素C含量对圆斑星鲽幼鱼抗氨氮胁迫能力的影响。试验在（12.5±1.5）℃的水温下进行,选择体重为（38.0±0.8）g的健康圆斑星鲽幼鱼,随机分为7组（每组3个重复,每个重复30尾）,分别投喂维生素C含量为10.2（对照）、249.1、402.8、616.2、769.5、909.4和1 177.8 mg/kg的试验饲料8周。投喂试验结束后,从每个重复取10尾鱼,用20 mg/L的氨氮胁迫24 h。结果表明：无论是氨氮胁迫前还是氨氮胁迫后,在饲料维生素C含量达到769.5 mg/kg时,肝脏和肌肉中维生素C积累达到饱和,再继续升高饲料维生素C含量,肝脏、肌肉中维生素C含量不再显著升高（P〉0.05）。除1 177.8 mg/kg维生素C组外,无论是氨氮胁迫前还是氨氮胁迫后,各维生素C添加组圆斑星鲽幼鱼血清中过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性均显著高于对照组（P〈0.05）。除616.2 mg/kg维生素C组血清中CAT活性无显著变化（P〉0.05）外,氨氮胁迫使各组血清中CAT和SOD活性显著降低（P〈0.05）,但添加维生素C可以减少降低的幅度。无论是氨氮胁迫前还是氨氮胁迫后,各维生素C添加组鳃丝Na^＋/K^＋-ATP酶活性均显著高于对照组（P〈0.05）。氨氮胁迫使对照组及909.4和1 177.8 mg/kg维生素C组鳃丝Na^＋/K^＋-ATP酶活性显著降低（P〈0.05）,其他组则无显著变化（P〉0.05）。氨氮胁迫使各组血清中葡萄糖和乳酸含量显著升高（P〈0.05）,对照组血清中皮质醇含量显著升高（P〈0.05）。此外,氨氮胁迫使各组血清中总铁结合力显著降低（P〈0.05）。综合各项测定指标,圆斑星鲽幼鱼饲料中维生素C含量在402.8~616.2 mg/kg时可有效提高机体抗氨氮胁迫能力。Ammonia-nitrogen pollution is one of the important pollutants in aquaculture,thus improving the resistance of pollution stress of the fish is very important.This experiment using different vitamin C content diets to feed juvenile spotted halibut（Verasper variegatus）,in order to get the effects of dietary vitamin C content on anti-ammonia-nitrogen stress ability of juvenile spotted halibut.Water temperature was maintained at（12.5±1.5）℃ in this experiment.Juvenile spotted halibut with the body weight of（38.0±0.8） g were randomly assigned into 7 groups with 3 replicates per group and 30 fish per replicate,and fed 7 experimental diets with the vitamin C content was 10.2,249.1,402.8,616.2,769.5,909.4 and 1 177.8 mg/kg,respectively.The experiment lasted for 8 weeks.After feeding trial,each replicate selected 10 fish to stress 24 h by 20 mg/L ammonia-nitrogen.The results showed as follows：whether before or after ammonia-nitrite stress,the vitamin C accumulation in muscle and liver reached saturation when dietary vitamin C content was 769.5 mg/kg,and continued to rise the dietary vitamin C content,the vitamin C content in muscle and liver was not significantly increased（P〉0.05）.The activities of serum catalase（CAT） and superoxide dismutase（SOD） in vitamin C addition groups except 1 177.8 mg/kg vitamin C group were significantly higher than those in control group whether before or after ammonia-nitrite stress（P〈0.05）.The activities of serum CAT and SOD in groups except serum CAT activity in 616.2 mg/kg vitamin C group were significantly decreased by ammonia-nitrite stress（P〈0.05）,and decline ranges in vitamin C addition groups were less compared with control group.The gill Na^＋/K^＋-ATPase activity in vitamin C addition groups was significantly higher than that in control group whether before or after ammonia-nitrite stress（P〈0.05）.The gill Na^＋/K^＋-ATPase activity in control group and 909.4,1 177.8 mg/kg vitamin C groups was significantly decreased（P〈0.05）,bu

关 键 词：圆斑星鲽幼鱼 维生素C 应激反应 抗氨氮胁迫 

分 类 号：S963[农业科学—水产养殖]