引药米酒对瓜蒌薤白中3,29-二苯甲酰基栝楼仁三醇血浆蛋白结合率的影响  

The effect of rice wine as cites drug on plasma protein binding rate of 3,29-dibenzoyl-karounitriol in Gualou-Xiebai

作　　者：邹纯才[1] 蒿艳 鄢海燕[1] 杨维成[1] 张敏杰[1] ZOU Chuncai HAO Yan YAN Haiyan YANG Weicheng ZHANG Minfie(Pharmacy School, Wannan Medical College, Wuhu 241002, China)

机构地区：[1]皖南医学院药学院,安徽芜湖241002

出　　处：《药学研究》2016年第12期687-689,699,共4页Journal of Pharmaceutical Research

基　　金：安徽高校省级自然科学研究重大项目(No.KJ2015ZD41;No.KJ2016SD60);安徽省科技攻关项目(No.12010402089);国家大学生创新创业训练计划项目(No.201510368019)

摘　　要：目的探讨引药米酒对瓜蒌薤白中3,29-二苯甲酰基栝楼仁三醇血浆蛋白结合率的影响。方法采用平衡透析法利用高效液相色谱技术测定瓜蒌薤白米酒溶液和水溶液中的3,29-二苯甲酰基栝楼仁三醇(质量浓度为0.8、1.6、3.2μg·m L-1)的血浆蛋白结合率。高效液相色谱条件为:YMC-Pack ODS-A色谱柱(4.6 mm×250 mm,5μm),甲醇-水(93∶7)为流动相,DAD检测器,检测波长为231 nm。结果瓜蒌薤白米酒溶液和水溶液中的3,29-二苯甲酰基栝楼仁三醇在大鼠、家兔、人血浆中的血浆蛋白结合率较高,没有种属差异和质量浓度依赖性。结论引药米酒未对瓜蒌薤白中3,29-二苯甲酰基栝楼仁三醇血浆蛋白结合率产生影响。Objective To study the effects of rice wine as cites drug on the plasma protein binding rate of 3,29- dibenzoyl-karounitriol in Gualou-Xiebai.Methods The plasma protein binding rate of 3,29-dibenzoy1-karounitriol （ mass concentrations were 0.8,1.6,3.2 μg · mL^-1） in Gualou-Xiebai rice wine solution and aqueous solution were determined by equilibrium dialysis method, and the concentration of 3,29-dibenzoyl-karounitriol was assayed by HPLC.HPLC conditions： ODS-A YMC-Pack column（ 4.6 mm×250 mm, 5 μm） , methanol water （ 93 ： 7 ） as mobile phase, DAD detector, detection wavelength was 231 nm.Results The plasma protein binding rate of 3,29-dibenzoyl-karounitriol in Gualou-Xiebai rice wine solution and aqueous solution was higher with no species difference and mass concentration dependence.Conclusion Rice wine as cites drug had no effect on the plasma protein binding rate of 3,29-dibenzoyl karounitriol in Gualou-Xiebai.

关 键 词：引药米酒 瓜蒌薤白 3 29-二苯甲酰基栝楼仁三醇 血浆蛋白结合率 

分 类 号：R285[医药卫生—中药学]