小檗碱对甲型流感病毒FM1株感染小鼠肺中RLH信号通路的影响  

Effect of Berberine on RLH Signaling Pathway in Lung of Mice Infected by Influenza A(FM1 strain)

作　　者：张梦媛[1] 颜宇琦[1] 秦洪琼 王森林[1] 施珊珊[1] 吴莎[1] 江振友[1] ZHANG Meng-yuan YAN Yu-qi QIN Hong-qiong WANG Sen-lin SHI Shan-shan WU Sha JIANG Zhen-you(Department of Microbiology and Immunology, School of Medicine, and Guangdong Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou 510632, China.)

机构地区：[1]暨南大学医学院微生物与免疫学系//广东省分子免疫学与抗体工程重点实验室,广东广州510632

出　　处：《中山大学学报（医学科学版）》2016年第6期834-839,共6页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金(81473454;81473557);中央高校基本科研业务费专项基金(21615416);广东省自然科学基金(2015A030313331)

摘　　要：【目的】研究小檗碱对甲型流感病毒FM1株感染小鼠肺组织中RLH信号通路的影响,探讨其抗病毒作用的机制。【方法】以50μL流感病毒FM1株LD50滴鼻感染RIG-I-/-小鼠和正常野生型C57BL/6小鼠,建立小鼠流感模型,分别用小檗碱和奥司他韦治疗。观察小鼠的生存率以及肺组织炎症损伤状况;RT-q PCR和Western-blot检测正常野生型C57BL/6小鼠RLH信号通路中RIG-I、NF-κB和MAVS的表达。【结果】正常野生型C57BL/6小鼠小檗碱组的生存率为80%,与病毒组50%相比明显提高,其肺组织病变局限,炎症明显减轻。通过RT-q PCR和Western-blot检测到小檗碱可下调正常野生型C57BL/6小鼠RLH信号通路中RIG-I、NF-κB和MAVS的表达。与病毒组相比,RIG-I、NF-κB和MAVS的m RNA表达量分别降低42.28%、33.12%和48.12%,同时,它们的蛋白表达量分别降低17.37%、13.83%和28.91%。【结论】小檗碱对于FM1株引起的小鼠流感具有较好的治疗作用,可通过影响RLH信号传导通路的活化来发挥抗流感病毒作用。[Objective]To determine the effect of berberine on RLH signaling pathway infected by influenza A （FM1 strain） in mice, and explore its anti-influenza mechanism. [ Methods ] RIG-I-/- mice and normal wide-type C57BL/6 mice were intranasally inoculated with 50 μL LD50 influenza A （FM1 strain）, and treated with berberine and osehamivir. The survival rates and pathological changes in the lung tissue after infection were examined. Using real-time fluorescence quantitative PCR reaction （RT-qPCR） and Western-blot to detect the RIG-I, NF-κB and MAVS expression of normal wide-type C57BL/6 mice. [ Results] The berberine could protect the normal wide-type C57BL/6 mice from the viral infection and reduce the pathological degree of the viral pneumonia. In addition, the survival rate of berberine group increased from 50% to 80% compared with virus group. RT-qPCR experiments and Western-blot experiments showed that berberine could reduce RIG-I, NF-κB and MAVS expression of virus-infected normal wide- type C57BL/6 mice. Compared with virus group, the expression of their mRNA reduced 42.28%, 33.12% and 45.12% separately and the expression of protein reduced 17.37%, 13.83% and 28.91% separately. [Conclusions] The berberine had good effect on prohibit influenza A virus as oseltamivir, which could regulate RLH signaling pathway of the infection.

关 键 词：小檗碱 RLH信号通路 流感病毒FM1株 炎症反应 

分 类 号：R392.5[医药卫生—免疫学]