外周血单个核细胞EB病毒负荷的荧光定量PCR检测在评估儿童传染性单核细胞增多症病情中的临床意义  

Clinical significance on condition assessment of Epstein-Barr viral load in peripheral blood mononuclear cells detected by quantitative PCR in children with infectious mononucleosis

作　　者：王惠霞 WANG Hui-xia.(Department of Pediatrics, Northwest Women And Children Hospital, Xian, Shanxi 710061 China)

机构地区：[1]西北妇女儿童医院儿科,陕西西安710061

出　　处：《中国优生与遗传杂志》2016年第12期34-36,20,共4页Chinese Journal of Birth Health & Heredity

摘　　要：目的探讨外周血单个核细胞（PBMC）EB病毒负荷的荧光定量聚合酶链反应（PCR）检测在评估儿童传染性单核细胞增多症（IM）病情中的临床意义。方法选取2014年12月~2016年8月入住我院的IM患儿80例,于患儿起病7d内应用荧光定量PCR检测患儿PBMC及血浆中EB病毒DNA,按照PBMC中EB病毒DNA负荷量分为高病毒量组和低病毒量组。结果 80例患儿中,PBMC中EB病毒DNA检出阳性率为90.00%（72/80）,血浆EB病毒DNA检出阳性率为42.50%（34/80）,差异具有统计学意义（P〈0.01）;高病毒量组中重度肝脏肿大、中重度脾脏肿大分布率明显高于低病毒量组（P〈0.01）;高病毒量组患儿ALT、AST、CK、CK-MB水平均明显高于低病毒量组患儿（P〈0.001）;高病毒量组患儿热退时间、肝脾肿大消退时间、住院时间均明显长于低病毒量组患儿（P〈0.001）。结论应用荧光定量PCR检测PBMC中EB病毒DNA可作为IM早期诊断方法,较血浆标本EB病毒DNA检出阳性率更高,同时也可用于患儿病情的评估。Objective：To investigate the clinical significance on condition assessment of Epstein-Barr viral load in peripheral blood mononuclear cells（PBMC）detected by quantitative polymerase chain reaction（PCR）in children with infectious mononucleosis（IM）. Methods：80 cases of children with IM in our hospital between December 2014 to August 2016 were selected,and the Epstein-Barr viral load in PBMC and plasma were detected by quantitative PCR within 7d of onset. Children with IM who detected positively for the Epstein-Barr viral in PBMC were divided into high viral load group and low viral load group according to Epstein-Barr viral load in PBMC. Results：80 cases of children,the positive rate of Epstein-Barr viral load in PBMC detected by quantitative PCR was 90.00%（72/80）,and Epstein-Barr viral load in plasma was 42.50%（34/80）,with statistically significant difference（P〈0.01）;The distribution ratio of moderate to severe enlargement of the liver,spleen of high viral load group was significantly higher than that of low viral load group（P〈0.01）;The ALT,AST,CK,CK-MB levels of high viral load group were significantly higher than that of low viral load group（P〈0.001）;The time of antipyretic,hepatosplenomegaly subsided,hospital stay of high viral load group were significantly longer than that of low viral load group（P〈0.001）.Conclusion：Epstein-Barr viral load in PBMC detected by quantitative PCR can be used as an early diagnostic methods for children with IM,and has higher positive rate than detected Epstein-Barr virus DNA in plasma samples,but also can be used to assess condition.

关 键 词：传染性单核细胞增多症 EB病毒DNA 外周单个核细胞 荧光定量聚合酶链反应 病情评估 

分 类 号：R725.1[医药卫生—儿科]