高效液相色谱法测定复方鱼腥草颗粒中黄芩苷和黄芩素含量  

Determination of Baicalin and Baicalein in Compound Yuxingcao Granule by High-performance Liquid Chromatography

作　　者：匡钊 

机构地区：[1]江苏省扬州市食品药品检验检测中心,江苏扬州225009

出　　处：《安徽中医药大学学报》2016年第6期91-94,共4页Journal of Anhui University of Chinese Medicine

摘　　要：目的建立同时测定复方鱼腥草颗粒中黄芩苷和黄芩素含量的方法。方法采用高效液相色谱法，以Thermo-C18（4．6mm×250mm，5μm）为色谱柱，以乙腈-0．1％甲酸溶液为流动相，进行梯度洗脱，流速为1．0mL／min，进样量为10μL，检测波长为274nm，柱温为25℃。结果可在30min内完成对黄芩苷和黄芩素的色谱分析，测得的黄芩苷的线性范围为0．069-0．34mg／mL，r=0．9998，平均回收率为99．5％（RSD=3．33％）；黄芩素的线性范围为0．005～0．025mg／mL，r=0．9994，平均回收率为102．7%（RSD=2．449／6）。结论所建立的高效液相色谱法方便快捷、准确、简便、重复性好，可用于复方鱼腥草颗粒的质量控制。Objective To establish a method for simultaneous determination of baicalin and baicalein in compound Yuxingcao Granule. Methods High-performance liquid chromatography （HPLC） was per- formed on a Thermo-C18 column （4. 6 mm×250 mm, 5 μm） with a mobile phase of acetonitrile-0. 1% formic acid for gradient elution at a flow rate of 1.0 mL/min, a sample size of 10μL, a detection wavelength of 274 nm, and a column temperature of 25℃. Results The chromatographic analysis of baicalin and baicalein was completed within 30 minutes. Baicalin and baicalein showed a good linear relationship within the ranges of 0. 069-0. 34 mg/mL（r=0. 999 8） and 0. 005-0. 025 mg/mL （r=0. 999 4）, respectively, with average recovery rates of 99. 5% （RSD=3. 33%） and 102. 7% （RSD=2. 44%）, respectively. Conclusion The HPLC method established is quick, accurate, and convenient and has good repeatability. Therefore, it can be used for quality control of compound Yuxingcao Granule.

关 键 词：复方鱼腥草颗粒 黄芩苷 黄芩素 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]