牛粪发酵沼液中产甲烷菌的分离及分子鉴定  

Molecular Identification of Methanogens Isolated from Biogas Slurry Fermented with Cow Dung

作　　者：陈楠楠[1,2] 叶丽丽[1] 王华欣[1] 祝婧[1] 乔波[1] 赵静虎 刘通[1] 岳山[1] 周金玲[1] 刘宇[1,2] 朱战波[1] Chen Nannan Ye Lili Wang Huaxin Zhu Jing Qiao Bo Zhao Jinghu Liu Tong Yue Shan Zhou Jinling Liu Yu Zhu Zhanbol(College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319 Veterinary Science Research Institute of Heilongjiang Province)

机构地区：[1]黑龙江八一农垦大学动物科技学院,大庆163319 [2]黑龙江省兽医科学研究所

出　　处：《黑龙江八一农垦大学学报》2016年第6期117-121,共5页journal of heilongjiang bayi agricultural university

基　　金：国家科技支撑计划课题(2013BAD21B01-1)

摘　　要：为了确定牛粪发酵沼液中疑似菌株的生物特性和种属关系。采用Hungate滚管技术、厌氧培养、革兰氏染色镜检、PCR扩增技术和抗生素耐受性鉴定等方法进行分析。实验结果表明,牛粪发酵沼液分离到了一株革兰氏阳性菌,能够利用CO_2,最适生长温度为37℃,单独或成对存在。菌落呈灰白色,光滑,表面凸起。PCR扩增出1 200 bp的16S rDNA片段,测序结果表明,该分离菌为产甲烷短杆菌。Biological characteristics and relationship between species of Methanogens in biogas slurry fermented with cow dung were researched by Hungate rolling-tube technique,anaerobic culture method, Gram＇s method,PCR and Antibiotic resistance identification. Results showed that a gram-positive isolated bacteria was able to take advantage of the CO2,growth temperature was 37 ℃ ,existed alone or in pairs. Colonis were pale,smooth and convex. PCR results showed that 16SrDNA fragments of 1 200 bp were amplified,isolated bacteria was identified as Methanogens by DNA sequencing technology.

关 键 词：产甲烷菌 厌氧培养 沼气 

分 类 号：S216.4[农业科学—农业机械化工程]