合成大麻素JWH-122的高效液相色谱分析方法研究  

Study on the analytic method of synthetic cannabinoid JWH-122 by the high performance liquid chromatography

作　　者：苗翠英[1] 钱尊磊 张林峰[1] 翟晚枫[2] Miao Cuiying Qian Zunlei Zhang Linfeng Zhai Wanfeng(College of Forensic Science, People＇s Public Security University of China, Beijing 100038, China Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China)

机构地区：[1]中国人民公安大学刑事科学技术学院,北京100038 [2]公安部物证鉴定中心,北京100038

出　　处：《中国法医学杂志》2016年第6期563-566,共4页Chinese Journal of Forensic Medicine

摘　　要：目的研究被我国《非药用类麻醉药品和精神药品列管办法》列入管制的合成大麻素JWH-122的高效液相色谱分析方法。方法以甲醇-去离子水(50%-50%)为流动相进行梯度洗脱,考查有机相初始浓度、梯度陡度、柱温、流速等色谱条件及检测波长,确定最优实验条件,在优化条件下对线性范围及专属性进行实验,通过实际样本检测对所建方法进行验证。结果紫外光谱检测波长221nm、有机相初始浓度为70%、梯度陡度为0.5%/min、流速1.2ml/min、柱温30℃条件下JWH-122在0.002mg/ml-0.1mg/ml范围内线性良好,检出限(S/N≥3)为0.1μg/ml;实际样本检测表明,优化条件下JWH-122能与样本中其它组分很好分离。结论该方法具有快速、灵敏、准确、分离效果好的优点,适用于新型香料毒品中合成大麻素JWH-122的分析检测。Objective To establish an effective method for determination of synthetic cannabinoid JWH-122 by the high-performance liquid chromatography （HPLC）, which is controled by China＇s Regulations on non-clinical narcotic and psychoactive drug. Methods Methanol-deionized water （50%-50%） was used as mobile phase for gradient elution. In addition to the initial concentration in organic phase, gradient steepness, column temperature, flow rate and other chromatographic conditions, the determine wavelengths were tested so as to find out optimal experimental conditions. Linearity range and specificity were tested under optimal conditions, and actual samples were used to verify the method established. Results Under the condition of ultraviolet spectrum detection wavelength at 221nm, initial concentration of 70%, organic phase gradient steepness of 0.5%/min, flow rate at 1.2 ml/min and column temperature of 30℃ ,excellent linearity of JWH-122 was observed at 0.002mg/mL-0.1mg/mL and the detection limit （S/N ≥ 3） was 0.1μg/mL. The test of actual samples suggested that JWH-122 was able to be well separated from the sample under the optimal conditions. Conclusion Our method has advantages of rapidity, sensitivity, accuracy and excellent separation efficiency, and is capable of the detection of synthetic cannabinoid JWH-122 of the novel ＂spice＂ drugs.

关 键 词：法医毒物分析 合成大麻素 JWH-122 高效液相色谱 

分 类 号：D919[医药卫生—法医学]