超滤管在陈旧生物检材中的应用  

The application of ultrafiltration in old biological samples

作　　者：谷建立[1] 廖燕妮[1] 梁婷 王燕[1] 曾琴 Gu Jianli Liao Yanni Liang Ting Wang Yan Zeng Qin(Nangchang Forensic Science and Technology Institute, Jiangxi Nanchang 330038, China Jiangxi Zhongzheng Forensic Expertise Institution, Jiangxi Nanchang 330002, China)

机构地区：[1]南昌市公安局刑事科学技术研究所,江西南昌330038 [2]江西中正司法鉴定中心,江西南昌330002

出　　处：《中国法医学杂志》2016年第6期607-609,共3页Chinese Journal of Forensic Medicine

摘　　要：目的探讨millipore超滤管滤过方法对陈旧生物检材DNA分型检验的应用价值。方法将23份陈旧血样分别剪取同样合适大小的血片3组,标为A、B、C组,磁珠法提取DNA,分别用80μL、80μL、20μL洗脱液洗脱得到模板DNA,其中A、C组模板直接扩增,B组用millipore超滤管滤过浓缩后扩增。PCR产物用AB3130x L基因分析仪检测,Gene Mapper ID V3.2软件进行自动分型。所得实验数据用SPSS软件分析处理。结果 A组没有1例样本扩出全部STR基因座,B组有18例样本扩出全部STR基因座,C组有11个样本扩出全部STR基因座。结论应用millipore超滤管滤过方法可以明显提高陈旧生物检材的DNA分型成功率。Objectve To investigate the application value of ultrafiltration with millipore filtration method test of old biological samples DNA. Methods 23 old blood samples separately were cut blood piece equally suitable size of three groups, labeled A, B and C group. DNA was extracted by magnetic bead methed and get DNA template with 80μL, 80μL, 20μL elution solution, respectively, then the DNA template in group A and C take right amount directly amplified, group B with milipore ultrafiltration filtration condensed amplication. PCR products were detected by ABI3130xl genetic analyzer and analyzed by GeneMapperID V3.2 software. The date were analyzed by using SPSS software. Results No samples were amplified for all STR loci in group A. There were 18 cases in group B samples amplified all STR loci and 11 samples in group C. Conclusion Application of millipore ultrafiltration method can significantly improve the old biological samples DNA classification success rate.

关 键 词：法医物证学 超滤管 陈旧生物检材 DNA分型 

分 类 号：D919.4[医药卫生—法医学]