肺胀炎性因子变化规律随机平行对照研究  

Lung Distension Inflammatory Factor Variation Random Parallel Control Study

作　　者：韦衮政[1] 韦爱欢[2] 石聪颖[3] 王现[3] 祁燕 

机构地区：[1]云南省中医医院肺病科,昆明650021 [2]柳州市中医院心病科,柳州545001 [3]云南中医学院临床医学院,昆明650021 [4]云南省高校中医药临床科研重点实验室,昆明650021

出　　处：《实用中医内科杂志》2016年第12期4-9,共6页Journal of Practical Traditional Chinese Internal Medicine

基　　金：云南省教育厅科研基金(NO:2013Y233)~~

摘　　要：[目的]观察肺胀炎性因子变化规律。[方法]使用随机平行对照方法,将95例住院患者随机分为三组。辨证分型:肺肾气虚、痰热郁肺、阳虚水泛,并以健康志愿者21名作为正常对照组。采用酶联免疫吸附测定法检测血清TNF-α、IL-8、AQP_2、AVP、BNP、Na^+-K^+-ATPase,SPSS17.0统计软件分析检测结果。计量资料用均数±标准差(x±s)表示,正态分布用Kolmogorov-Smirnov和Shapiro-Wilk检验;多组均数正态分布采用多因素方差分析,非正态分布用两个独立样本秩和检验,相关分析比较变量相关性。[结果]肺肾气虚与痰热郁肺及痰热郁肺与阳虚水泛TNF-α有显著性差异(P<0.05)。肺肾气虚与痰热郁肺IL-8有显著性差异(P<0.05)。肺肾气虚与阳虚水泛AVP有显著性差异(P<0.05)。痰热郁肺与阳虚水泛Na^+-K^+-ATPase有显著性差异(P<0.05)。肺肾气虚AQP2与IL-8呈显著性负相关(P<0.05);AQP_2与BNP呈显著性正相关(P<0.01);AQP_2与AVP呈显著性正相关(P<0.05);IL-8与BNP呈显著性负相关(P<0.05);IL-8与TNF-α呈显著性正相关(P<0.05);BNP与TNF-α呈显著性负相关(P<0.05);AQP_2与TNF-α呈显著性负相关。痰热郁肺AQP_2与BNP呈显著性正相关(P<0.01);IL-8与TNF-α呈显著性正相关(P<0.01);Na^+-K^+-ATPase与AVP呈显著性正相关(P<0.01)。阳虚水泛AQP_2与IL-8呈显著性正相关(P<0.05);AQP_2与BNP呈显著性正相关(P<0.01);AQP_2与Na^+-K^+-ATPase呈显著性正相关(P<0.05);IL-8与Na^+-K^+-ATPase呈显著性正相关(P<0.01)。[结论]⑴TNF-α是肺胀急性发作期的关键指标,也是肺胀实证与虚证的鉴别点。⑵IL-8是肺胀肺肾气虚与痰热郁肺之间的区别点,是肺肾气虚的关键指标。⑶AVP是肺肾气虚与阳虚水泛之间的鉴别点。⑷Na^+-K^+-ATPase在肺胀阳虚水泛出现抑制或耗竭现象,在肺肾气虚、痰热郁肺还可被动激活。⑸肺肾气虚AQP_2随着BNP、AVP同向增高,AQP_2、BNP随着IL-8、TNF-α的激活而被抑制或耗竭。水转运及心衰因[Objective] Observe lung distension inflammatory factors. [Methods] Using random parallel control method, 95 cases of hospitalized patients were randomly divided into three groups. Syndrome differentiation type：lung and kidney deficiency, phlegm hot yu lung, Yang deficiency water flooding. As normal control group and 21 cases in healthy volunteers. Using enzyme-linked immunosorbent assay to detect the serum TNF- α ,IL-8, AQP2, AVP, BNP, Na＋-k＋-ATPase level. Using SPSS17.0 statistical software analysis of test results. Measurement data with mean ＋ standard deviationx ＋ s） said that during normal distribution with the Kolmogorov Smirnov has and Shapiro Wilk inspection, more sets of normally distributed mean comparison using multi-factor analysis of variance, not normally distributed multiple sets of mean is used when comparing two independent sample rank and inspection, compare the correlation between variables with the method of correlation analysis. [Results] Lung and kidney deficiency and phlegm hot yu lung and phlegm hot yu lung syndrome and Yang deficiency water flooding TNF- α level have obvious difference（P〈0.05）. Lung and kidney deficiency and phlegm hot yu lung IL-8 levels have obvious difference（P〈0.05）. Lung and kidney deficiency and Yang deficiency water flooding AVP level have obvious difference（P〈0.05）.Phlegm hot yu lung and Yang deficiency water flooding Na^＋-k^＋-ATPase have obvious difference（P〈0.05）. Lung and kidney deficiency AQP2 and IL-8 has a significant negative correlation（P〈0.05）; AQP2 has significant positive correlation with the BNP（P 〈0.01）; AQP2 and AVP has a significant positive correlation（P〈0.05）; IL-8 has significant negative correlation with the BNP（P〈0.05）; IL-8 and TNF- ot has a significant positive correlation（P〈0.05）; The BNP has significant negative correlation with the TNF- ct （P〈0.05）; AQP2 and TNF- et has a significant negative correlation. Phlegm hot yu lung AQP2 has significant positive correlati

关 键 词：肺胀 肿瘤坏死因子-α 白介素-8 水通道蛋白-2 脑钠素 精氨酸加压素 钠钾三磷酸腺苷酶 

分 类 号：R541.4[医药卫生—心血管疾病]