MULTI-TILLERING DWARF1, a new allele of BRITTLE CULM 12, affects plant height and tiller in rice  

作　　者：Haiping Yu Deyong Ren Yangzhou Zhu Jiangmin Xu Yuexing Wang Ruifang Liu Yunxia Fang Zhenyuan Shi Jiangjie Pan Mei Lu Bojun Ma Jiang Hu Yuchun Rao Haiping Yu;Deyong Ren;Yangzhou Zhu;Jiangmin Xu;Yuexing Wang;Ruifang Liu;Yunxia Fang;Zhenyuan Shi;Jiangjie Pan;Mei Lu;Bojun Ma;Jiang Hu;Yuchun Rao(College of Chemistry and Life Sciences, Zhejiang Normal University;State Key Laboratory for Rice Biology, China National Rice Research Institute;Key Laboratory of Northeast Rice Biology and Breeding,Ministry of Agriculture, and Key Laboratory of Northern Japonica Rice Genetics and Breeding, Ministry of Education,Rice Research Institute, Shenyang Agricultural University)

机构地区：[1]College of Chemistry and Life Sciences, Zhejiang Normal University [2]State Key Laboratory for Rice Biology, China National Rice Research Institute [3]Key Laboratory of Northeast Rice Biology and Breeding,Ministry of Agriculture, and Key Laboratory of Northern Japonica Rice Genetics and Breeding, Ministry of Education,Rice Research Institute, Shenyang Agricultural University

出　　处：《Science Bulletin》2016年第23期1810-1817,共8页科学通报（英文版）

基　　金：supported by the National Natural Science Foundation of China (31401464, 31201183);Zhejiang Provincial Natural Science Foundation of China (Y3110194, LY16C130001);China Postdoctoral Science Foundation (2014M561108);the Open Foundation from Zhejiang Provincial Top Key Discipline of Biology (KFJJ2014006)

摘　　要：Plant height and tillering are crucial factors determining rice plant architecture and influencing rice grain production. In this study, rnulti-tillering dwarf1 （mtdl）, a stable multi-tiller and dwarf mutant, was screened from the ethylmethane sulfonate-treated japonica rice variety Wuyunging7. Compared with the wild type, mtdl mutant exhibited pleiotropic phenotypes, including dwarf- ism, more tillers, brittle culms and delayed heading date. By employing map-based cloning strategy, the gene MTD1 was finally mapped to an approximately 66-kb region on the short arm of chromosome 9. Sequencing results showed that the gene LOCOsO9g02650 （BC12） in mtdl mutant had a single nucleotide substitution （G to A）, which gen- erated a premature translation stop. Over-expressing MTD1/BC12 coding sequ（nce rescued all the phenotypes of mtdl mutants including plant height and tillers, which confirms that BC12 is the mutated gene in mtdl mutant. Quantitative reverse tran,-eription-PCR analysis showed that MTDI/BCI2 could negatively regulate the expression of MONOCULM 1, IDEAL PLANT ARCHITECTURE1 and Tillering and Dwarf 1, and control rice tillering. Remark- ably, a-amylase activity analysis and gibberellic acid （GA） treatment showed that the dwarf phenotype of mtdl mutant was dependent on GA biosynthesis pathway. These results facilitated to further uncover the molecular mechanism of the growth and development in rice.Plant height and tillering are crucial factors determining rice plant architecture and influencing rice grain production. In this study, multi-tillering dwarf1(mtd1), a stable multi-tiller and dwarf mutant, was screened from the ethylmethane sulfonate-treated japonica rice variety Wuyunging7. Compared with the wild type, mtd1 mutant exhibited pleiotropic phenotypes, including dwarfism, more tillers, brittle culms and delayed heading date.By employing map-based cloning strategy, the gene MTD1 was finally mapped to an approximately 66-kb region on the short arm of chromosome 9. Sequencing results showed that the gene LOC_Os09g02650(BC12) in mtd1 mutant had a single nucleotide substitution(G to A), which generated a premature translation stop. Over-expressing MTD1/BC12 coding sequence rescued all the phenotypes of mtd1 mutants including plant height and tillers, which confirms that BC12 is the mutated gene in mtd1 mutant.Quantitative reverse transcription-PCR analysis showed that MTD1/BC12 could negatively regulate the expression of MONOCULM 1, IDEAL PLANT ARCHITECTURE1 and Tillering and Dwarf 1, and control rice tillering. Remarkably, a-amylase activity analysis and gibberellic acid(GA)treatment showed that the dwarf phenotype of mtd1 mutant was dependent on GA biosynthesis pathway. These results facilitated to further uncover the molecular mechanism of the growth and development in rice.

关 键 词：Rice - Dwarf Tillering - mtdl Gene cloning 

分 类 号：S511[农业科学—作物学] Q347[生物学—遗传学]