高表达CCL20对乙肝相关慢加急性肝衰竭患者的影响  

Influence of CCL20 Overproduction on Patients with HBV Related Acute-on-chronic Liver Failure

作　　者：张耿林[1] 徐士丞 朱翔[1] 谢婵[1] 彭亮[1] 高志良[1] ZHANG Geng-lin XU Shi-cheng ZHU Xiang XIE Chan PENG Liang GAO Zhi-liang(Department of infectious diseases Department of Ultrasound, The Third Affiliated Hospital of Sun-Yat-Sen University, Guangzhou510630, China)

机构地区：[1]中山大学附属第三医院感染病科,广东广州510630 [2]中山大学附属第三医院超声科,广东广州510630

出　　处：《中山大学学报（医学科学版）》2016年第5期692-696,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家"十三五"科技重大专项(2016ZX10002008;2016ZX10002009;2016ZX10002010);广州市科技计划项目(1561000155)

摘　　要：【目的】:探讨乙肝相关慢加急性肝衰竭(HBV-ACLF)患者血清CCL20的表达及其高表达对HBV-ACLF的影响。【方法】收集30例HBV-ACLF患者外周血,以30例慢性乙型肝炎(CHB)患者和27例健康体检者(NC)作为对照,采用ELISA法检测血清中CCL20的表达,荧光定量PCR法检测HBV-DNA定量,化学发光法检测HBe Ag的状态及甲胎蛋白水平,分析CCL20的表达与HBV病毒产物及临床指标的关系。【结果】与NC组及CHB组相比,HBV-ACLF患者血清CCL20水平明显升高(P<0.001)。通过相关分析,发现高表达CCL20与总胆红素成正相关关系(P=0.048);同时发现高表达CCL20与甲胎蛋白水平成正相关关系(P=0.021)。低HBV-DNA复制患者其CCL20水平高于HBV-DNA较高复制组(P=0.044),然而不同HBe Ag状态的HBV-ACLF患者其CCL20水平差异无统计学意义。【结论】CCL20趋化因子参与了HBV-ACLF疾病的发生发展,且CCL20高表达提示患者具有良好肝细胞再生潜力。[ Objective ] To investigate serum CCL20 level and its influence on the patients with HBV related acute-on-chronic liver failure （HBV-ACLF）. [Methods] Peripheral blood samples were collected from 30 patients with HBV-ACLF, 30 patients with chronic hepatitis B （CHB） and 27 normal controls （NC）. Serum CCL20 level was measured using ELISA. HBV-DNA loads were measured by fluorescent PCR. The presence of HBeAg and AFP levels were measured by automated chemiluminescence assay. Serum CCL20 levels in HBV-ACLF patients and their correlations with HBeAg presence, HBV-DNA loads and liver function were analyzed. [ Results ] Serum CCL20 levels were significantly elevated in patients with HBV-ACLF compare with NC subjects and CHB patients （P 〈 0.001 ）. CCL20 levels were positively correlated with Tbil levels （P = 0.048） and AFP levels （P=0.021）. Higher CCL20 levels were found in patients with lower HBV-DNA loads than those with higher HBV-DNA loads （P=0.044）. However, no significant differences in CCL20 levels were observed between HBeAg-positive and HBeAg-negative patients with HBV-ACLF. [Conclusion] CCL20 chemokine may contributed to the mechanism of HBV-ACLF, and overproduction of CCL20 may indicate latent capacity of hepatocyte regenaration.

关 键 词：乙型肝炎病毒 慢加急性肝衰竭 CCL20 

分 类 号：R512.6[医药卫生—内科学]