ABO疑难血型的血清学与PCR-SSP检测分析  

Serological and PCR-SSP detection analysis of suspicious ABO blood group

作　　者：乔艳辉[1] 郭伟鹏[1] 木耶赛尔.伊斯马依力 徐保红[1] 

机构地区：[1]乌鲁木齐市血液中心血型室,乌鲁木齐830000

出　　处：《临床血液学杂志（输血与检验）》2016年第6期954-955,958,共3页Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)

基　　金：新疆维吾尔自治区自然科学基金(No:2015211A009)

摘　　要：目的:通过对ABO疑难血型的血清学与基因检测分析,探讨ABO亚型的分布状况和ABO血型基因分型的意义。方法:采用试管法正反定型、吸收放散试验等一系列血型血清学方法检测分析97例正反定型不符血液标本的血清学表型,对其中61例标本,辅以聚合酶链式反应-特异性序列引物(PCR-SSP)基因分型技术检测其基因型。结果:97例ABO疑难样本中检出A亚型13例,B亚型8例,AB亚型50例,B(A)18例、CisAB 5例,Bh m 1例,O型抗-B抗体缺失1例,1例有直抗阳性和自身抗体干扰,排除后被定为A型。PCR-SSP技术检出常见ABO基因4个(A、A201、B、O),以及ABO基因型6个(A/O、A201/O、B/O、A/B、A201/B、O/O)。结论:ABO疑难血型的鉴定应采用多种血清学方法进行检测,PCR-SSP技术能识别ABO基因型,作为一种辅助方法可应用于检测ABO疑难血型。Objective：To investigate the distribution of ABO subgroups and the significance of ABO genotyping through serological and PCR-SSP detection analysis of suspicious ABO blood group.Method：The traditional tube typing test,sorption-elution and series serological tests were used to determine the serological phenotypes of 97 samples for ABO discrepancy,and 61 specimens of them were geneotyped by polymerase chain reaction with sequence-specific primers（PCR-SSP）.Result：In these 97 cases,13cases were subtypes of A,8cases were subtypes of B,50 cases were subtypes of AB,18 cases were B（A）group,5cases were CisAB group,1case was Bhm,1case was group O with anti-B missing,and 1case was group A after eliminating the interference of autoantibodies and DAT positive.PCR-SSP found 4common ABO alleles and 6ABO genotypes.Conclusion：Blood group identification of specimen with difficult ABO blood group should be checked by a variety of serological methods.PCR-SSP could identify ABO blood group genotype,as an auxiliary method,it could be used to detect ABO typing discrepancies.

关 键 词：ABO亚型 血清学 PCR-SSP基因分型 

分 类 号：R457.1[医药卫生—治疗学]