具有高抗胃肠道胁迫嗜酸乳杆菌的筛选及对肠炎小鼠的治疗作用  

Screening of Lactobacillus acidophilus of high resistance against gastro-intestinal tract and the immunomodulating effect of its on artificial ulcerative colitis mice

作　　者：徐敏[1] 蒙月月 靳妲 李娜[1] 闫芬芬[1] 霍贵成[1] XU Min MENG Yue-yue JIN Da LI Na YANG Fen-fen HUO Gui-cheng(Northeast Agriculture University, Key Laboratory of Dairy Science, Ministry of Education, Harbin 150030, China)

机构地区：[1]东北农业大学,乳品科学教育部重点实验室,黑龙江哈尔滨150030

出　　处：《食品工业科技》2017年第1期354-359,共6页Science and Technology of Food Industry

基　　金：国家自然科学基金(31401512);国家863项目:乳酸菌特色资源库及乳酸菌发酵剂和代谢工程技术研究(2011AA100902)

摘　　要：目的:从三株嗜酸乳杆菌中筛选出具有高抗胃肠道胁迫特性的嗜酸乳杆菌并研究其对溃疡性结肠炎小鼠的治疗效果。方法:人工模拟人体消化道胃液、肠液和胆盐环境,设置不同浓度梯度,研究三株嗜酸乳杆菌进入人体后对在消化道胃液、肠液和胆盐环境中的存活率,从而反映嗜酸乳杆菌对人体消化道胃液、肠液和胆盐环境的抵抗力。然后以筛选出的具有高抗胃肠道胁迫能力的嗜酸乳杆菌为研究对象,让处理组小鼠自由饮用3%葡聚糖硫酸钠盐(DSS)溶液7 d,以人工诱发的溃疡性结肠炎(UC)小鼠为动物模型,观察此株高性能的嗜酸乳杆菌CGMCC10436的抗溃疡性结肠炎潜力。结果表明,嗜酸乳杆菌CGMCC10436在模拟胃酸pH2.5~4.5时,其活菌数大于10~7CFU/mL,并且在3 h时没有下降的趋势;在模拟肠液中2h时,嗜酸乳杆菌CGMCC10436活菌数有所上升;并且嗜酸乳杆菌CGMCC10436在胆盐浓度为4g/kg时,3 h时其活菌数大于1×107CFU/mL。嗜酸乳杆菌CGMCC10436发挥较好的抗溃疡性结肠炎功效的剂量为3×108CFU,能够改善溃疡性结肠炎小鼠表观状况,修复溃疡性结肠炎小鼠肠粘膜损伤,增强溃疡性结肠炎小鼠的免疫调节。Objective ：To screen the Lactobacillus acidophilus of the high resistance against gastro-intestinal tract from three Lactobacillus acidophilus and study it＇s immunomodulating effect on artificial ulcerative colitis mice.Methods：The environment of gastric juice, intestinal juice and bile salt were simulated, different concentration were set, and survival rates of three Lactobacillus acidophilus in the environment of gastric juice,intestinal juice and bile salt were studied to reflect the resistance against gastro-intestinal tract of human.Lactobacillus acidophilus with a high resistance to the gastrointestinal tract was studied as the object, and mice from the treated groups were freely drunken 3% dextran sulfate sodium salt （DSS）solution for 7 days and were studied as the animal model to study the anti-colitis potential .Results showed that, when the pH of stomach acid was at 2.5-4.5, the bacterial count of Lactobacdlus acidophilus CGMCC10436 was greater than 10^7 CFU/mL, and there was no downward trend at 3 h. In the environment of intestinal juice for 2 h, the bacterial count of Lactobacillus acidophilus CGMCC10436 was greater.At a concentration of 4 g/kg in the environment of bile salt, the bacterial count is greater than the 1 ×10^7 CFU/mL at 3 h.Lactobacillus acidophilus CGMCC10436 had high resistance against gastro-intestinal tract, and 3 ×10^7 CFU Lactobacillus acidophilus CGMCC10436 could have anti-inflammatory effect,repaired the injury of intestinal mucosa and enhanced immune regulation.

关 键 词：嗜酸乳杆菌 抗胃肠道胁迫能力 溃疡性结肠炎 

分 类 号：TS201.4[轻工技术与工程—食品科学]