机械应力改变致印度豪猪蛋白在早期骨关节炎软骨下骨的表达  

Research of the expression of subchondral bone of Indian hedgehog with early experimental osteoarthritis induced by mechanical stress

作　　者：张荣凯[1] 李国威[1] 张大卫[1] 于兵[1] 冯韶燕[2] Zhang Rongkai Li Guowei Zhang Dawei Yu Bing Feng Shaoyan(Department of Orthopedics, the Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai 519000, China)

机构地区：[1]中山大学附属第五医院骨外科,广东省珠海市519000 [2]中山大学附属第五医院耳鼻喉科,广东省珠海市519000

出　　处：《中华医学杂志》2017年第1期53-56,共4页National Medical Journal of China

基　　金：广东省医学科研基金（A2014261）,广东省自然科学基金（2015A030310240）

摘　　要：目的研究关节不稳导致的机械应力改变引起的早期骨关节炎软骨下骨的印度豪猪蛋白（Ihh）表达情况，为阐述其在骨关节炎致病中的重要作用提供依据。方法大鼠分为实验组和对照组各15只。实验组切除右膝内侧半月板及内侧副韧带，对照组仅切开关节囊。于术后1、2、4周取右膝关节标本，采用免疫组化及免疫荧光技术分析关节软骨下骨破骨细胞Ihh的表达情况。结果术后1、2周，与对照组比较，实验组软骨下骨区检测到大量的Ihh强阳性多核巨细胞；术后4周实验组、对照组均未检测到Ihh阳性细胞。术后1、2、4周，实验组阳性区域平均光密度（A）分别为（0．351±0．086）、（0．303±0．026）、（0．092±0．033）；对照组平均A分别为（0．153±0．017）、（0．176±0．013）、（0．136±0．014）；术后1、2周两组比较差异有统计学意义（P〈0．5）；术后4周两组比较差异无统计学意义（P〉0．5）。以破骨细胞的标志蛋白Trap与Ihh进行免疫荧光双重染色实验表职Ihh强阳性多核巨细胞为破骨细胞。结论软骨下骨破骨细胞Ihh的表达可能在早期创伤性骨关节炎致病机制中起了重要作用。Objective To investigate the the expression of subchondral bone of Indian hedgehog （Ihh） with early experimental osteoarthritis induced by mechanical stress. Methods The animals were equally divided into two groups： experimental group（ E-group, n = 15 ） and control group（ S-group, n = 15 ）. The right knee joints of E-group underwent surgery, which involved in both medial collateral ligament （MCL） transaction and medial meniscectomy, while the control group was only carried out with a sham operation. The rats were killed at 1, 2 and 4 weeks postsurgery to obtain the right knee joints. Immunostaining and immunofluorescence double staining were performed to evaluate the expression of Ihh in subchondral bone, respectively. Results The polynuclear giant cells in the subchondral bone of E-Group expressed Ihh in their cytoplasm 1 and 2 weeks post-surgery, except for 4 week, while those of S-Group appeared negative at all three time points postsurgery. There is statistically difference between the mean density of positive area of Ihh in sections of E-group and S-group both in 1 week （E-group：0. 351 ±0. 086, S-group ：0. 153 ± 0. 017, P 〈 0. 05） and 2 weeks （ E-group ： 0. 303 ± 0. 026, S-group ： 0. 176 ± 0. 013, P 〈 0. 05 ）, but without statistically difference in 4 weeks （E-group： 0. 092 ± 0. 033, S-group： 0. 136 ± 0. 014, P 〉 0. 05 ） post surgery. Trap positive giant cells in subehondral bone of E-group were also found Ihh positive,which indicated expression of Ihh in osteoclast. Conclusion Ihh maybe play an important role in pathogenesis of early experimental osteoarthritis.

关 键 词：骨关节炎 软骨 关节 应力 物理 印度豪猪蛋白 

分 类 号：R684.3[医药卫生—骨科学]