MicroRNA的超灵敏检测研究进展  

Advance in Ultrasensitive Detection of MicroRNA

作　　者：王子月[1] 刘萌[1] 张春阳[1] WANG Ziyue LIU Meng ZHANG Chunyang(College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China)

机构地区：[1]山东师范大学化学化工与材料科学学院,济南250014

出　　处：《高等学校化学学报》2017年第1期1-11,共11页Chemical Journal of Chinese Universities

基　　金：国家杰出青年科学基金(批准号:21325523);科技部重点领域创新团队项目(批准号:2015RA4024)资助~~

摘　　要：综述了最新发展的MicroRNA(miRNA)分析方法,包括比色、荧光、化学发光、表面增强拉曼、单分子检测和电化学分析,并对miRNA检测的发展方向做了展望.MicroRNA （miRNA） is a class of endogenous noncoding RNA molecule with the length of about 22 nucleotides. MicroRNA may regulate more than 50% of human protein-coding genes, and plays important roles in regulating cell differentiation and other biological processes. The abnormal expression of miRNA is closely related to the occurrence of many diseases including cancers. Therefore, miRNA may function as a potential biomarker for molecular diagnostics. The sensitive detection of miRNA is essential to the early clinical diagnosis and the development of anticancer drugs. Because of the short size, low abundance in total RNA samples and the homologous similarities in miRNA family, the ultrasensitive detection of miRNA remains a great challenge. Recently, many new methods have been developed to improve the detection sensitivity. This review focuses on the advance in miRNA detection methods including colorimetric assay, fluorescent assay, ehemiluminescent assay, surface enhanced Raman scattering, single molecules detection and electrochemical detection. In addition, we give a new insight into future development of miRNA assay.

关 键 词：MICRORNA 超灵敏检测 扩增 分析方法 

分 类 号：O655[理学—分析化学]