开胸法检测肺高血压小鼠模型右心室血液动力学指标  

A novel open chest method of right ventricular hemodynamics measurement in pulmonary hypertensive mice

作　　者：袁安慧[1] 何阳阳[1] 杨淑慧[1] 李素琪[1] 叶珏[1] 胡帅[1] 邱家勇 荆志成[1] 王晓建[1] Yuan Anhui He Yangyang Yang Shuhui Li Suqi Ye Jue Hu Shuai Qiu Jiayong Jing Zhicheng Wang Xiaojian(State Key Laboratory of Cardiovascular Diseases, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China)

机构地区：[1]中国医学科学院北京协和医学院国家心血管病中心阜外医院心血管疾病国家重点实验室,100037 [2]河南科技大学临床医学院河南科技大学第一附属医院呼吸科

出　　处：《中华心血管病杂志》2016年第12期1036-1039,共4页Chinese Journal of Cardiology

基　　金：国家自然科学基金（81300184,81320108005）;北京市自然科学基金（7141009）

摘　　要：目的右心导管法检测小鼠右心室血液动力学指标，操作时间长，成本高且成功率低。本研究旨在建立一种呼吸机辅助的开胸直视操作检测小鼠右心室血液动力学指标的方法，并用以评估小鼠病理生理条件下的血液动力学状态。方法雄性8周龄C57BL／6小鼠16只，采用完全随机设计法将小鼠分为对照组和低氧组，每组8只。低氧组小鼠在模拟海拔5500m环境的低压低氧舱（氧浓度10％）内持续饲养，对照组小鼠饲养于常压常氧环境中。3周后对两组小鼠进行血液动力学检测，气管插管，开胸，采用0．7mm×19mm密闭式留置针，从心尖处进针5mm测量右心室压力。结果开胸法检测小鼠右心室血液动力学指标，从气管插管到获得右心室血流参数耗时5～10min，成功率100％。对照组和低氧组小鼠在麻醉状态下心率差异无统计学意义[（306．4±11．5）次／min比（320．4±16．0）次／min，P〉0．05]。低氧组小鼠右心室收缩压高于对照组[（22．6±1．0）mmHg（1mmHg=0．133kPa）比（17．1±1．0）mmHg，P〈0．01]，右心室平均压高于对照组[（12．4±0．3）mmHg比（9．6±0．8）mmHg，P〈0．01]，右心室内压最大上升速度和下降速度的绝对值均显著大于对照组[（639．7±47．7）mmHg/s比（421．3±30．6）mmHg／s和（-496．5±40．6）mmHg／s比（-324．3±24．0）mmHg／s，P均〈0．01]。结论开胸方法检测小鼠右心室血液动力学指标，操作简便、成功率高，可以很好地反映小鼠在不同病理生理条件下的血液动力学状态。Objective Right heart catheterization is the gold-standard for measurement of right ventricular hemodynamics. However, it is difficult to perform in mice. We thus aimed to establish a new open chest method to record and analyze the right ventricular hemodynamic parameters in intubated mice connected to a respirator under anesthesia. Methods Eight-week old male C57BL/6 mice were divided into the control group, and the hypoxia group using completely random method. Mice in the hypoxia group were exposed in low pressure oxygen chamber, which simulated the environment of plateau （5 500 m altitude, 10% oxygen ） for 3 weeks. Mice in the control group were exposed in normal pressure and oxygen environment. The mice were intratracheally intubated and connected to a respirator, chest was opened, right ventricular hemodynamics were measured with a needle （0. 7 mm × 19 mm） punctured into right ventricle （5 mm in death）, which was connected to the measuring equipment. Results The open chest procedure could be finished within 5 - 10 minutes. The successful rate was 100%. There was no significant difference in heart rate between control group and hypoxia group under anesthetia （ （306. 4 ± 11.5 ） bpm vs. （320. 4 ± 16.0） bpm, P〉 0.05）. Compared with the control group, both the right ventricular systolic pressure （RVSP） and the mean right ventricular pressure （mRVP）were increased in hypoxia group （（ 17.1 ± 1.0）mmHg（ 1 mmHg =0. 133 kPa） vs. （22.6 ± 1.0） mmHg,P 〈0. 01 ; （9. 6 ±0. 8） mmHg vs. （ 12. 4 ± 0. 3） mmHg,P 〈0. 01 ）. Moreover, the absolute value of the maximal rate of increase in right ventrieular pressure （dp/dtmax） and the maximal rate of decrease in right ventricular pressure （dp/dtmin） were higher in the hypoxia group （ （ 421.3 ± 30. 6 ） mmHg,/s vs. （ 639.7 ± 47.7 ） mmHg/s, P 〈 0. 01 ; （ - 324. 3 ± 24. 0 ） mmHg/s vs, （ - 496. 5 ± 40. 6 ） mmHg/s, P 〈 0.01 ）, indicating the pathological status after hypoxia treatment. Co

关 键 词：高血压 肺性 模型 动物 血液动力学现象 

分 类 号：R544.1[医药卫生—心血管疾病] R-332[医药卫生—内科学]