苯磺酸左旋氨氯地平对腹主动脉缩窄大鼠心肌能量代谢的影响  

The Effect of Levamlodipine on the Myocardial Energy Metabolism in Rats Subjected to Abdominal Aortic constriction

作　　者：陈爱兰[1] 罗福全[1] 林峰丞 范婷婷[2] 陈新宇[3] 何志裕[1] 王慧勇[1] CHEN Ailan LUO Fuquan LIN Fengcheng FAN Tingting CHEN Xinyu HE Zhiyu WANG Huiyong(The Cardiovascular Meicine of the First Affiliated Hosiptal of Guangzhou Medical University, Guangzhou, Guangdong 510120, China The Clinical Laboratory of the First Affiliated Hosiptal of Guangzhou Medical University, Guangzhou, Guangdong 510120, China Department of Pathogenic Biology of Guangzhou Medical University, Guangzhou , Guangdong 511436, China)

机构地区：[1]广州医科大学附属第一医院心血管内科,广东广州510120 [2]广州医科大学附属第一医院检验科,广东广州510120 [3]广州医科大学病原生物学教研室,广东广州511436

出　　处：《今日药学》2016年第11期766-769,共4页Pharmacy Today

基　　金：广东省药学会科学研究基金(2013X33);广州市科信局基金(2014Y2-00085);广东省自然科学基金(2014A030310077);广东省高等学校优秀青年教师培养计划资助项目(YQ2015138);广州医科大学博士启动基金(2013C33)

摘　　要：目的探讨苯磺酸左旋氨氯地平对腹主动脉部分缩窄大鼠心肌能量代谢的影响及其机制。方法将18只Wistar大鼠随机分为3组:假手术组(Sham组)、手术组(AAC组)和手术+苯磺酸左旋氨氯地平治疗组(AAC+LA组),采用腹主动脉部分缩窄法建立压力超负荷致心肌肥厚的动物模型,AAC+LA组每天给予苯磺酸左旋氨氯地平2.5 mg·kg^(-1)(溶于2 m L的生理盐水中),Sham组及AAC组给予等体积的生理盐水;8周后,测量全心/体质量的比值;颈总动脉插管检测血压;小动物超声测量心脏功能;采用荧光定量(QT-PCR)法检测心肌组织GLUT4 m RNA表达,试剂盒测定心肌组织ADP/ATP比值。结果 8周后与Sham组比较,AAC组血压、全心/体质量比值、左室壁厚度、ADP/ATP比值增加,心脏功能、GLUT4表达下降;AAC+LA组血压、全心/体质量比值、左室壁厚度、ADP/ATP比值下降,心脏功能改善,GLUT4表达上升。结论苯磺酸左旋氨氯地平具有改善压力负荷大鼠心肌收缩、舒张功能的作用,其机制可能与其降低血压的同时增加肥厚心肌GLUT4的表达、改善心肌的能量代谢有关。OBJECTIVE To investigate the effect and mechanism of levamlodipine on the myocardial energy metabolism in rats subjected to abdominal aortic constriction. METHODS 18 Wistar rats were randomly divided into 3 groups： sham operated group（ Sham group）,abdominal aortic constriction group（ AAC group） and AAC＋Levamlodipine treatment group（ AAC＋LA group）. The pressure overload induced cardiac hypertrophy models was established by using the abdominal aortic constriction method. LA group was administered levamlodipine 2.5 mg·kg^（-1）·d^（-1）,dissolved in 2 m L of saline,Sham group and AAC group received an equal volume of saline.After 8 weeks,the ratio of heart / body weight was measured,the blood pressure was examined by using carotid artery catheter.small animal ultrasound was used to measure cardiac function. the GLUT4 m RNA expression of myocardium was detected by real-time quantitative PCR. The ADP / ATP ratio was examined by Using assay kit. RESULTS After 8 weeks,compared with Sham group,blood pressure,heart / body weight ratio,left ventricular wall thickness and ADP / ATP ratio of AAC group increased. Cardiac function and GLUT4 expression decreased.Compared with AAC group,blood pressure,heart / body weight ratio,left ventricular wall thickness and ADP / ATP ratio of AAC ＋ LA group decreased,cardiac function improved and GLUT4 expression increased. CONCLUSION Levamlodipine improved cardiac systolic and diastolic function in pressure overload myocardium,which may be related to decrease the blood pressure while increase the expression of GLUT4 and improve myocardial energy metabolism.

关 键 词：苯磺酸左旋氨氯地平 腹主动脉缩窄 心肌 能量代谢 大鼠 

分 类 号：R961[医药卫生—药理学] R972[医药卫生—药学]