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作 者:禹松林[1] 程倩[1] 韩建华[1] 周伟燕[2] 程歆琦[1] 侯立安[1] 高冉[1] 苏薇[1] 李志 邱玲[1]
机构地区:[1]中国医学科学院北京协和医院检验科,100730 [2]北京医院临床检验中心 [3]赛默飞世尔科技有限公司北京应用部
出 处:《中华检验医学杂志》2016年第12期917-921,共5页Chinese Journal of Laboratory Medicine
基 金:国家临床重点专科,国家自然科学基金(81201337)
摘 要:目的:建立电感耦合等离子体质谱法( ICP-MS)检测人体尿液和血清中碘的方法,以用于临床实验室评估人体碘营养状态。方法方法学验证。采用氨水∶异丙醇∶水作为稀释剂,10倍稀释样本,稀释混匀后采用ICP-MS检测尿液或血清中碘,以铼作为内标。评价ICP-MS方法的线性、检测限、回收率、准确度、精密度、携带污染及稳定性。通过回顾性分析45例妊娠妇女尿液和血清中碘含量,评价正常孕妇体内碘摄入情况。结果该方法分析样本时间仅用30 s,检出限为0.87μg/L,线性相关系数大于0.9999,血清及尿液中加样回收率均在100%左右(95.3%~109.9%)。正确度良好,和美国NIST尿液标准物质SRM3668相比偏倚均小于4%(-0.9%~3.9%)。血清中碘的重复性变异为1.2%~3.0%,实验室内变异为3.0%~3.8%;尿液中碘的重复性变异为2.0%~2.9%,实验室内变异为4.1%~4.9%。平均携带污染率为0.03%。在-20℃及4℃下放置一个月,碘结果均相对稳定。正常孕妇体内尿碘浓度为154.8±89.7μg/L(均值±SD),血清碘浓度为75.8±21.4μg/L,两者相关系数R2为0.21。结论本研究成功建立了ICP-MS高通量快速测定尿液和血清碘的方法,该方法准确、特异,适用于临床大样本量分析。Objective This paper aims at establishing a inductively coupled plasma mass spectrometry ( ICP-MS) method for quantification and evaluation of iodine in human urine and serum in routine clinical laboratory .Methods This study was methodology validation research on iodine evaluation using ICP-MS.Ammonia, isopropanol and ultrapure water were mixed at certain ratio to dilute samples in the ratio of 1:10, and then the diluted samples were analyzed by ICP -MS.Re was used as the internal standard.And linearity, lower limit of detection, recovery, precision, accuracy, carryover and stability was evaluated thoroughly .Results of iodine of pregnant women who required iodine tests were retrospectively analyzed to evaluate the status of iodine .Results The method only needs 30s for analysis of one sample .It was sensitive with a lower limit detection of 0.87μg/L, the correlation coefficient was higher than 0.999 9 in ten measurements.The recovery in both serum and urine was approximately 100% (95.3% -109.9%). Based on the NIST standard reference material 3668 comparison, the bias was less than 4%( -0.9% -3.9%).The inter-coefficient variation (CV) for serum iodine and urine iodine was 1.2%-3.0%, 2. 0%-2.9%, respectively;and total CV for serum iodine and urine iodine were 3.0%-3.8%, 4.1%-4.9%, respectively.The mean carryover of this method was 0.03% and iodine was stable for at least one month at -20℃ and 4℃.The urine and serum iodine for pregnant women was (154.8 ±89.7) μg/L (mean ±SD),(75.8 ±21.4) μg/L, respectively.The correlation between urine and serum iodine was 0.21. Conclusion Establishe a rapid and simple ICP -MS method for urine and serum iodine measurement with high accurate and precise in routine clinical laboratory .
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