痛风泰颗粒对急性痛风大鼠IL-1、IL-6及TNF-α含量的影响  

Effect of Tongfengtai Granules on Contents of IL-1,IL-6,TNF-α in Acute Gout Rat

作　　者：张剑勇[1] 王辉[2] 谢静静[1] 肖敏[1] 李丹[1] 

机构地区：[1]深圳市中医院,广东深圳518033 [2]深圳市宝安区中医院,广东深圳518100

出　　处：《新中医》2016年第12期201-203,共3页New Chinese Medicine

基　　金：深圳市科技研发资金项目(JCYJ20130329155553735)

摘　　要：目的:观察痛风泰颗粒对急性痛风大鼠炎症因子白细胞介素-1(IL-1)、白细胞介素-6(IL-6)及肿瘤坏死因子-α(TNF-α)含量的影响,探讨痛风泰颗粒治疗痛风的可能作用机理。方法:将36只SD大鼠随机分为空白组、模型组、痛风泰颗粒组、戴芬组,除空白组外,其他三组建立急性痛风性关节炎的病理模型。造模成功后,分别药物干预7天,观察各组实验大鼠造模前后关节肤色、肤温、肿胀等情况,应用酶联免疫法(ELISA)检测大鼠右内踝关节液中的IL-1、IL-6的含量和血清中TNF-α含量。结果:与空白组比较,模型组IL-1、IL-6、TNF-α的含量显著升高(P<0.05),提示急性痛风模型造模成功。与模型组比较,痛风泰颗粒组和戴芬组IL-1、IL-6、TNF-α的含量显著下降,差异均有统计学意义(P<0.05)。痛风泰颗粒组与戴芬组比较,差异无统计学意义。结论:痛风泰颗粒可能是通过抑制炎症因子IL-1、IL-6、TNF-α的释放来治疗痛风。Objective：To obeserve the effect of Tongfengtai granules on contents of Interleukin-1（IL-1）,Interleukin-6（IL-6）,Tumor Necrosis Factor-α（TNF-α） in acute gout rat,discuss possible Mechanism.Methods：Thirty-six SpragueDawley（SD） rats were divided into the blank group,model group,tongfengtai granules group,difene group randomly.Except blank group,the other three groups were induced to pathological model with acute gouty arthritis.After successful modeling,seven-day drug intervention was given separately.Observed joint skin tone,skin temperature,swelling of experimental rats.Applyied euzymelinked immunosorbent assay（ELISA） to test IL-1、IL-6 contents in right ankle joint fluid,and TNF-αcontents in serum.Results：Compared with the blank group,contents of IL-1,IL-6,and TNF-α in model group were increased significantly（P〈0.05）,suggesting the success of acute gout model.Compared with the model group,contents ofIL-1,IL-6,and TNF-α in tongfengtai granules group and difene group were decreased significantly（P〈0.05）.Compared tongfengtai granules group with difene group,the difference was not statistically significant（P〈0.05）.Conclusion：Tongfengtai granules may treat gout by inhibiting release of inflammation factor IL-1,IL-6 and TNF-α.

关 键 词：痛风泰颗粒 急性痛风 白细胞介素-1(IL-1) 白细胞介素-6(IL-6) 肿瘤坏死因子-α(TNF-α) 动物实验 大鼠 

分 类 号：R285.5[医药卫生—中药学]