HPLC法测定紫背天葵中矢车菊素-3-O-葡萄糖苷含量的研究  

Research on Content Determination of Procyanidin-3-O-glucoside in Begonia Fimbristipula Hance with HPLC

作　　者：邵考珍 何淑莹 

机构地区：[1]佛山市南海区第七人民医院药剂科,广东佛山528247

出　　处：《新中医》2016年第12期204-206,共3页New Chinese Medicine

摘　　要：目的:建立紫背天葵中矢车菊素-3-O-葡萄糖苷含量测定的高效液相色谱法(HPLC)。方法:采用Shim-pack VPODS(250 mm×4.6 mm,5μm)为色谱柱,0.5%磷酸-50%乙腈为流动相;检测波长为280 nm;流速0.8 mL/min;柱温30℃。结果:矢车菊素-3-O-葡萄糖苷在0.05μg^1μg的范围内与峰面积呈良好的线性关系(r=0.999 9),平均加样回收率为100.4%,RSD为1.3%(n=6)。结论:该方法可准确测定紫背天葵中矢车菊素-3-O-葡萄糖苷的含量,重复性好,可作为控制紫背天葵质量的一个重要指标。Objective：To establish high performance liquid chromatography（HPLC） method for content determination of Procyanidin-3-O-glucoside in Begonia Fimbristipula Hance.Methods：Adopted chromatographic column Shim-pack VP-ODS（250 mm×4.6 mm,5 μm,mobile phase was 0.5%Phosphoric acid-50%acetonitrile,detection wavelength was 280nm;flow rate was 0.8 mL/min and column temperature was 30℃）.Results：Procyanidin-3-O-glucoside and peak area were in a good linear relationship（r=0.999 9） in the range of 0.05 μg^1 μg.The average sample recovery rate was 100.4%,RSD（relative standard deviation） was 1.3%（n=6）.Conclusion：The therapy can determine contents of Procyanidin-3-O-glucoside in Begonia fimbristipula Hance accurately,with good repeatability.It＇s an important indicator to control the quality of Begonia fimbristipula Hance.

关 键 词：紫背天葵 HPLC法 矢车菊素-3-O-葡萄糖苷 含量测定 

分 类 号：R284[医药卫生—中药学]