大孔吸附树脂法富集钩藤散中抗老年痴呆有效成分实验研究  

Experiment Study on Enrichment of Anti-AD Effective Component in Gouteng San with Macroporous Resin

作　　者：郑肖熠 范征吟[2] 

机构地区：[1]宁波市镇海区招宝山街道社区卫生服务中心,浙江宁波315200 [2]上海市中医医院内分泌科,上海200040

出　　处：《新中医》2016年第12期214-216,共3页New Chinese Medicine

摘　　要：目的:探讨钩藤散大孔吸附树脂法不同洗脱物抗老年痴呆(AD)的作用。方法:采用大孔吸附树脂方法,水、20%乙醇、50%乙醇、70%乙醇、90%乙醇作为流动相依次对钩藤浸膏进行洗脱富集。60只大鼠中取10只设为对照组,剩余大鼠通过Aβ-淀粉样蛋白(Aβ1-40)注入大鼠脑室,建立AD大鼠模型。将成功造模的大鼠分为水洗脱物组、20%乙醇洗脱物组、50%乙醇洗脱物组、70%乙醇洗脱物组、90%乙醇洗脱物组各10只,将不同洗脱物分别予不同组AD大鼠给药,给药20天后,测定其电迷宫法行为学指标,考察各洗脱物对AD大鼠的记忆改善情况。结果:70%乙醇洗脱物组测试成绩与对照组比较,差异无统计学意义(P>0.05),提示钩藤70%乙醇洗脱物可以提升记忆能力,具有治疗老年痴呆的作用;其它洗脱物组大鼠行为测试成绩分别与对照组比较,差异均有统计学意义(P<0.05),提示其它洗脱物均不具有改善记忆的能力。结论:大孔吸附树脂法可有效分离富集钩藤的抗老年痴呆成分,70%乙醇洗脱物可有效改善记忆,具有潜在的抗老年痴呆作用。Objective：To discuss the effect of different components in Gouteng san on resisting Alzheimer＇s disease（AD）.Methods：By adopting the method of macroporous resin,water,20%ethanol,50%ethanol,70%ethanol,90%ethanol as mobile phase,elution and enrichment of Gouteng san were carried out in sequence,β-amyloid protein（Aβ1-40） was injected into ventricles of rats,and the AD rat model was established.Rats in different groups were given extractions of each component.Another ten rats were set as the blank group without any treatment.After giving medicine for 20 days,determined their behavior indexes in the electric maze and inspected the effect of each component on improving AD rats ＇memory.Results：Experiment suggested that in the 70%of ethanol extraction group,compared test performance of the blank group,there were no significant difference（P〈0.05）,which showed that Gouteng san extraction of 70%of ethanol can improve memory ability effectively and have an effect in treating AD.Rats＇ test performances in other groups were lower than those of normal rats（P〈0.05）,indicating that ethanol extractions of other gradients can not improve memory.Conclusion：Macroporous resin can effectively separate the anti-AD component in enriched Gouteng san.70%of ethanol extraction can improve memory effectively and have the potential for resisting AD.

关 键 词：钩藤散 老年痴呆症 大孔吸附树脂 

分 类 号：R284[医药卫生—中药学]